C57BL/6JCya-Qkiem1flox/Cya
Common Name:
Qki-flox
Product ID:
S-CKO-04643
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Qki-flox
Strain ID
CKOCMP-19317-Qki-B6J-VA
Gene Name
Product ID
S-CKO-04643
Gene Alias
1110003F05Rik; 1500005P18; Qk; l(17)-1Wis; l17Wis1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Qkiem1flox/Cya mice (Catalog S-CKO-04643) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000233645
NCBI RefSeq
NM_001159517
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Qki, also known as Quaking, is a member of the signal transduction and activation of RNA (STAR) family and the heterogeneous nuclear ribonucleoprotein K- (hnRNP K-) homology domain protein family. It plays diverse roles in regulating pre-mRNA post-transcriptional processing, intracellular migration, and mRNA stability. Qki is involved in multiple biological pathways and is of great biological importance, with its isoforms QKI-5, QKI-6, and QKI-7 having distinct functions in a cell-type-specific manner [5].
In various studies, gene knockout models have provided insights into Qki's functions. In lens-specific Qki-deficient mice, genes associated with cholesterol biosynthesis were downregulated, leading to a reduced cholesterol level in the eye lens, protein aggregate accumulation, and eventually cataracts. This shows Qki is required for the transcriptional activation of cholesterol biosynthesis genes in the eye lens [1]. In neonatal mice with Qki depletion in neural stem cells or oligodendrocyte precursor cells, cholesterol biosynthesis was impaired, resulting in defective myelinogenesis, highlighting Qki-Srebp2-controlled cholesterol biosynthesis as essential for myelinogenesis [2]. In microglia, deletion of the Qk gene severely impaired the clearance of myelin debris, indicating Qki is an essential regulator of microglial phagocytosis in demyelination [4]. In BMSCs-specific QKI-deficient mice, an increase in bone mass was observed, with promoted osteogenic differentiation, suggesting QKI suppresses osteogenic differentiation of BMSCs [3].
In conclusion, Qki is crucial for maintaining the cholesterol level in the eye lens to prevent cataracts, for myelinogenesis in the central nervous system, for microglial phagocytosis during demyelination, and for regulating osteogenic differentiation in bone marrow-derived mesenchymal stem cells. The study of Qki using gene knockout mouse models has significantly contributed to understanding its role in these biological processes and related diseases, such as cataracts, demyelinating diseases, and osteoporosis.
References:
1. Shin, Seula, Zhou, Hao, He, Chenxi, Chen, Yiwen, Hu, Jian. 2021. Qki activates Srebp2-mediated cholesterol biosynthesis for maintenance of eye lens transparency. In Nature communications, 12, 3005. doi:10.1038/s41467-021-22782-0. https://pubmed.ncbi.nlm.nih.gov/34021134/
2. Zhou, Xin, Shin, Seula, He, Chenxi, Lan, Fei, Hu, Jian. 2021. Qki regulates myelinogenesis through Srebp2-dependent cholesterol biosynthesis. In eLife, 10, . doi:10.7554/eLife.60467. https://pubmed.ncbi.nlm.nih.gov/33942715/
3. Yan, Zhao, Ruan, Banjun, Wang, Shan, Lu, Zifan, Cao, Xiaorui. 2023. RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway. In Archives of medical research, 54, 102853. doi:10.1016/j.arcmed.2023.102853. https://pubmed.ncbi.nlm.nih.gov/37460362/
4. Ren, Jiangong, Dai, Congxin, Zhou, Xin, Chen, Yiwen, Hu, Jian. . Qki is an essential regulator of microglial phagocytosis in demyelination. In The Journal of experimental medicine, 218, . doi:10.1084/jem.20190348. https://pubmed.ncbi.nlm.nih.gov/33045062/
5. Chen, Xinyun, Yin, Jianwen, Cao, Dayan, Liu, Ying, Shou, Weinian. 2021. The Emerging Roles of the RNA Binding Protein QKI in Cardiovascular Development and Function. In Frontiers in cell and developmental biology, 9, 668659. doi:10.3389/fcell.2021.668659. https://pubmed.ncbi.nlm.nih.gov/34222237/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen