C57BL/6JCya-Rab3aem1flox/Cya
Common Name:
Rab3a-flox
Product ID:
S-CKO-04658
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rab3a-flox
Strain ID
CKOCMP-19339-Rab3a-B6J-VA
Gene Name
Product ID
S-CKO-04658
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rab3aem1flox/Cya mice (Catalog S-CKO-04658) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000110093
NCBI RefSeq
NM_001166399
Target Region
Exon 2~4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Rab3a, a small GTP-binding protein, is a member of the Rab family implicated in intracellular vesicle trafficking. It is crucial for Ca(2+)-dependent exocytosis, such as neurotransmitter release from nerve terminals [4]. Rab3a is also involved in various biological processes including plasma membrane repair, neurite outgrowth, and atrial natriuretic peptide secretion [1,2,3].
In plasma membrane repair, Rab3a is essential for lysosome exocytosis. It forms a tripartite complex with synaptotagmin-like protein 4a and non-muscle myosin heavy chain IIA, which is vital for positioning peripheral lysosomes for repair [1]. In hippocampal neurons, Rab3a interacts with spastin, regulating its protein stability and microtubule-severing function to modulate neurite outgrowth [2]. In cardiomyocytes, zDHHC9 palmitoylates Rab3gap1, influencing Rab3a activity and atrial natriuretic peptide secretion [3]. Silencing the Rab3A/Rab27A system in podocytes affects differentiation, apoptosis, and cytoskeletal organization, highlighting its role in podocyte injury [5]. In a rat spinal cord injury model, Rab3a promoted the secretion of neuronal cell-derived extracellular vesicles by interacting with STXBP1, which inhibited M1 macrophage polarization and thus promoted spinal cord injury repair [6]. In melanocytes, RAB3A regulates melanin exocytosis and transfer induced by keratinocyte-conditioned medium [7]. In murine leukemia virus infection, Rab3a is required for the stabilization of the Gag protein [8]. Heterozygous RAB3A variants, like p.Arg83Trp, cause cerebellar ataxia through a partial loss-of-function mechanism [9].
In summary, Rab3a plays essential roles in multiple biological processes including vesicle-related functions such as exocytosis, secretion, and membrane repair. Research using various models, including those with genetic manipulations, has revealed its significance in diseases like spinal cord injury, podocyte-related injury, and cerebellar ataxia, providing insights into potential therapeutic targets for these conditions.
References：
1. Vieira, Otilia V. 2016. Rab3a and Rab10 are regulators of lysosome exocytosis and plasma membrane repair. In Small GTPases, 9, 349-351. doi:10.1080/21541248.2016.1235004. https://pubmed.ncbi.nlm.nih.gov/27687479/
2. Yang, Yuhao, Yang, Jie, Liang, Yaozhong, Lin, Hongsheng, Tan, Minghui. 2022. Rab3A interacts with spastin to regulate neurite outgrowth in hippocampal neurons. In Biochemical and biophysical research communications, 643, 77-87. doi:10.1016/j.bbrc.2022.12.066. https://pubmed.ncbi.nlm.nih.gov/36587525/
3. Essandoh, Kobina, Subramani, Arasakumar, Ferro, Olivia A, Koripella, Sribharat, Brody, Matthew J. 2023. zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1. In JACC. Basic to translational science, 8, 518-542. doi:10.1016/j.jacbts.2022.11.003. https://pubmed.ncbi.nlm.nih.gov/37325411/
4. Takai, Y, Sasaki, T, Shirataki, H, Nakanishi, H. . Rab3A small GTP-binding protein in Ca(2+)-dependent exocytosis. In Genes to cells : devoted to molecular & cellular mechanisms, 1, 615-32. doi:. https://pubmed.ncbi.nlm.nih.gov/9078389/
5. Martinez-Arroyo, Olga, Flores-Chova, Ana, Sanchez-Garcia, Belen, Cortes, Raquel, Ortega, Ana. 2023. Rab3A/Rab27A System Silencing Ameliorates High Glucose-Induced Injury in Podocytes. In Biology, 12, . doi:10.3390/biology12050690. https://pubmed.ncbi.nlm.nih.gov/37237503/
6. Yang, Yuhao, Liu, Ziqiao, Lu, Yang, Wang, Zemin, Zha, Dingsheng. 2024. Rab3a attenuates spinal cord injury by mediating vesicle release. In Brain research bulletin, 208, 110884. doi:10.1016/j.brainresbull.2024.110884. https://pubmed.ncbi.nlm.nih.gov/38253132/
7. Cabaço, Luís C, Bento-Lopes, Liliana, Neto, Matilde V, Seabra, Miguel C, Barral, Duarte C. 2022. RAB3A Regulates Melanin Exocytosis and Transfer Induced by Keratinocyte-Conditioned Medium. In JID innovations : skin science from molecules to population health, 2, 100139. doi:10.1016/j.xjidi.2022.100139. https://pubmed.ncbi.nlm.nih.gov/36090299/
8. Izumida, Mai, Kakoki, Katsura, Hayashi, Hideki, Matsuyama, Toshifumi, Kubo, Yoshinao. 2021. Rab3a, a small GTP-binding protein, is required for the stabilization of the murine leukaemia virus Gag protein. In Small GTPases, 13, 162-182. doi:10.1080/21541248.2021.1939631. https://pubmed.ncbi.nlm.nih.gov/34180342/
9. Hengel, Holger, Hannan, Shabab B, Reich, Selina, Schöls, Ludger, Synofzik, Matthis. 2025. Heterozygous RAB3A variants cause cerebellar ataxia by a partial loss-of-function mechanism. In Brain : a journal of neurology, , . doi:10.1093/brain/awaf111. https://pubmed.ncbi.nlm.nih.gov/40166812/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen