C57BL/6NCya-Rabggtbem1flox/Cya
Common Name:
Rabggtb-flox
Product ID:
S-CKO-04667
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rabggtb-flox
Strain ID
CKOCMP-19352-Rabggtb-B6N-VA
Gene Name
Product ID
S-CKO-04667
Gene Alias
--
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Rabggtbem1flox/Cya mice (Catalog S-CKO-04667) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000167111
NCBI RefSeq
NM_011231
Target Region
Exon 4~5
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Rabggtb, the β subunit of GGTase II, is a key component in protein prenylation, a post-translational modification. Protein prenylation is involved in membrane association and proper localization of proteins. It also regulates autophagy via Rab7 geranylgeranylation, which is crucial for the degradation of abnormal proteins in cells [1,2].
In the context of amyotrophic lateral sclerosis (ALS), a neurodegenerative disease, overexpression of Rabggtb via lentiviral transfection in NSC34-hSOD1G93A and TDP-43 cells improved cell proliferation by facilitating autophagosome-lysosome fusion. It also reduced the abnormal aggregation of SOD1 protein [1]. In SOD1G93A mice, overexpressing Rabggtb in spinal cord motoneurons via adeno-associated virus delayed the onset time and survival time, decreased SOD1 misfolding and glial overactivation [2]. Additionally, in ALS patients, the level of Rabggtb in monocytes and monocyte-derived macrophages was higher compared to healthy controls, and was significantly correlated with the ALSFRS-R and disease progression levels, suggesting it could be an indicator for tracking ALS progression [3,4].
In summary, Rabggtb is essential for protein prenylation-mediated autophagy. Studies in ALS-related cell and mouse models have revealed its potential therapeutic role in ALS, offering new insights into developing treatment strategies for this devastating neurodegenerative disease.
References:
1. Ma, Haiyang, Huo, Jia, Xin, Cheng, Li, Rui, Liu, Yaling. 2023. RABGGTB plays a critical role in ALS pathogenesis. In Brain research bulletin, 206, 110833. doi:10.1016/j.brainresbull.2023.110833. https://pubmed.ncbi.nlm.nih.gov/38042502/
2. Gao, Tianchu, Huo, Jia, Xin, Cheng, Li, Rui, Liu, Yaling. 2023. Protective effects of intrathecal injection of AAV9-RabGGTB-GFP+ in SOD1G93A mice. In Frontiers in aging neuroscience, 15, 1092607. doi:10.3389/fnagi.2023.1092607. https://pubmed.ncbi.nlm.nih.gov/36967828/
3. Yang, Jing, Xin, Cheng, Huo, Jia, Li, Rui, Liu, Yaling. 2023. Rab Geranylgeranyltransferase Subunit Beta as a Potential Indicator to Assess the Progression of Amyotrophic Lateral Sclerosis. In Brain sciences, 13, . doi:10.3390/brainsci13111531. https://pubmed.ncbi.nlm.nih.gov/38002490/
4. Yang, Jing, Tian, Mei, Zhang, Lei, Li, Rui, Liu, Yaling. 2024. Assessment of Rab geranylgeranyltransferase subunit beta in amyotrophic lateral sclerosis. In Frontiers in neurology, 15, 1447461. doi:10.3389/fneur.2024.1447461. https://pubmed.ncbi.nlm.nih.gov/39224887/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen