C57BL/6JCya-Rac1em1flox/Cya
Common Name:
Rac1-flox
Product ID:
S-CKO-04668
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rac1-flox
Strain ID
CKOCMP-19353-Rac1-B6J-VA
Gene Name
Product ID
S-CKO-04668
Gene Alias
D5Ertd559e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rac1em1flox/Cya mice (Catalog S-CKO-04668) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000080537
NCBI RefSeq
NM_009007
Target Region
Exon 2~3
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Rac1, also known as RAS-related C3 botulinum toxin substrate 1, is a key member of the Rho GTPases. It is a cytoskeleton regulation protein that participates in crucial cellular processes such as cell adhesion, morphology, movement, and also in pathways like regulation of oxidative stress, inflammatory and immune surveillance [2,4]. Rac1 is highly expressed in various tumors and is associated with tumorigenesis, progression, epithelial-mesenchymal transition, and metastasis [1,2,3,4,5,6].
Rac1 is frequently dysregulated and overexpressed in bladder pathologies, contributing to bacterial infections, diabetes-induced bladder dysfunctions, and bladder cancers [1]. Inhibitors like NSC23766 and EHT 1864 have shown activities against bladder cancer [1]. In metastatic cutaneous melanoma, RAC1 activation is related to cancer migration, invasion, angiogenesis, and metastasis, making it a potential target for therapy [6]. Also, Rac1 has been implicated in colorectal carcinogenesis, where its signaling pathway dysregulation occurs at different steps of neoplastic progression [5].
In conclusion, Rac1 is essential in multiple cellular functions and is closely associated with various diseases, especially cancers. The study of Rac1 through gene-knockout or conditional-knockout mouse models (if applicable in the references) could potentially provide deeper insights into its functions and its role in disease development, offering new strategies for disease treatment, such as in bladder and skin cancers [1,6].
References:
1. Sauzeau, Vincent, Beignet, Julien, Bailly, Christian. 2022. Rac1 as a Target to Treat Dysfunctions and Cancer of the Bladder. In Biomedicines, 10, . doi:10.3390/biomedicines10061357. https://pubmed.ncbi.nlm.nih.gov/35740379/
2. Liang, Jiaxin, Oyang, Linda, Rao, Shan, Zhou, Yujuan, Liao, Qianjin. 2021. Rac1, A Potential Target for Tumor Therapy. In Frontiers in oncology, 11, 674426. doi:10.3389/fonc.2021.674426. https://pubmed.ncbi.nlm.nih.gov/34079763/
3. De, Pradip, Aske, Jennifer Carlson, Dey, Nandini. 2019. RAC1 Takes the Lead in Solid Tumors. In Cells, 8, . doi:10.3390/cells8050382. https://pubmed.ncbi.nlm.nih.gov/31027363/
4. Bailly, Christian, Beignet, Julien, Loirand, Gervaise, Sauzeau, Vincent. 2022. Rac1 as a therapeutic anticancer target: Promises and limitations. In Biochemical pharmacology, 203, 115180. doi:10.1016/j.bcp.2022.115180. https://pubmed.ncbi.nlm.nih.gov/35853497/
5. Kotelevets, Larissa, Chastre, Eric. 2020. Rac1 Signaling: From Intestinal Homeostasis to Colorectal Cancer Metastasis. In Cancers, 12, . doi:10.3390/cancers12030665. https://pubmed.ncbi.nlm.nih.gov/32178475/
6. Colón-Bolea, Paula, García-Gómez, Rocío, Casar, Berta. 2021. RAC1 Activation as a Potential Therapeutic Option in Metastatic Cutaneous Melanoma. In Biomolecules, 11, . doi:10.3390/biom11111554. https://pubmed.ncbi.nlm.nih.gov/34827551/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen