C57BL/6JCya-Cnksr1em1flox/Cya
Common Name:
Cnksr1-flox
Product ID:
S-CKO-04706
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cnksr1-flox
Strain ID
CKOCMP-194231-Cnksr1-B6J-VA
Gene Name
Product ID
S-CKO-04706
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cnksr1em1flox/Cya mice (Catalog S-CKO-04706) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030645
NCBI RefSeq
NM_001081047
Target Region
Exon 2~3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Cnksr1, also known as connector enhancer of kinase suppressor of Ras 1, acts as a scaffold component for receptor tyrosine kinase in mitogen-activated protein kinase (MAPK) cascades. It is involved in regulating multiple cellular processes and signaling pathways, which are crucial for normal physiological functions and disease-related processes [2].
In HER2-positive breast cancer cells, degradation of RhoB by the CUL3/KCTD10 ubiquitin ligase complex is essential for EGFR and HER2 phosphorylation. RhoB-GTP and protein tyrosine phosphatase receptor type H (PTPRH) interact with CNKSR1. Constitutive RhoB degradation enables CNKSR1 to interact with PTPRH at the plasma membrane, inactivating EGFR phosphatase activity [1].
In pancreatic ductal adenocarcinoma, CNKSR1 is a mediator of resistance to MAPK (MEK) inhibition. MEK inhibition in CNKSR1high cancer cells leads to CNKSR1 translocation to the plasma membrane, where it interacts with and stabilizes phosphorylated AKT, promoting crosstalk to AKT signaling [3].
In an Iranian family with syndromic autosomal recessive intellectual disability, a novel frameshift mutation in CNKSR1 was detected. Lack of CNKSR1 transcripts and protein was observed in affected patients' lymphoblastoid cells, and RNAi-mediated knockdown of its Drosophila orthologue led to eye and mushroom body structure defects, suggesting its role in brain development [2].
In conclusion, Cnksr1 is vital for regulating signaling pathways such as MAPK and AKT, which are involved in cancer cell proliferation and resistance to treatment. Its role in brain development is also significant, as defects in Cnksr1 can lead to cognitive impairment. The study of Cnksr1 through various genetic models has provided valuable insights into these biological functions and disease-related mechanisms [1,2,3].
References：
1. Nishiyama, Kanako, Maekawa, Masashi, Nakagita, Tomoya, Takada, Yasutsugu, Higashiyama, Shigeki. 2021. CNKSR1 serves as a scaffold to activate an EGFR phosphatase via exclusive interaction with RhoB-GTP. In Life science alliance, 4, . doi:10.26508/lsa.202101095. https://pubmed.ncbi.nlm.nih.gov/34187934/
2. Kazeminasab, Somayeh, Taskiran, Ibrahim Ihsan, Fattahi, Zohreh, Najmabadi, Hossein, Kahrizi, Kimia. 2018. CNKSR1 gene defect can cause syndromic autosomal recessive intellectual disability. In American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, 177, 691-699. doi:10.1002/ajmg.b.32648. https://pubmed.ncbi.nlm.nih.gov/30450701/
3. Li, Dandan, Miermont, Anne M, Sable, Rushikesh, Hewitt, Stephen M, Rudloff, Udo. . Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling. In Molecular cancer research : MCR, 21, 316-331. doi:10.1158/1541-7786.MCR-21-1036. https://pubmed.ncbi.nlm.nih.gov/36790955/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen