C57BL/6JCya-Bex1em1flox/Cya
Common Name:
Bex1-flox
Product ID:
S-CKO-04780
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Bex1-flox
Strain ID
CKOCMP-19716-Bex1-B6J-VA
Gene Name
Product ID
S-CKO-04780
Gene Alias
Bex2; Bex4; Rex3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bex1em1flox/Cya mice (Catalog S-CKO-04780) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000058125
NCBI RefSeq
NM_009052
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Bex1, or brain-expressed X-linked protein 1, has diverse essential functions. It is involved in ciliogenesis, where it plays a crucial role in tubulin polymerization and primary cilia formation [3]. It also participates in pathways related to cell stemness, inflammation, and viral response. In terms of biological importance, it is associated with various diseases, especially liver cancer and heart-related diseases. Genetic models, such as knockout mice, have been instrumental in studying its functions.
In liver cancer, Bex1 is a novel cancer stem cell (CSC) marker. DNMT1-mediated methylation of Bex1 regulates the self-renewal of liver CSCs in hepatoblastoma (HB) and a subtype of hepatocellular carcinoma with high CSC features (CSC-HCC) through activation of the Wnt/β-catenin signaling pathway [1]. In HB, Bex1 promotes stemness maintenance by facilitating the Warburg effect in a PPARγ/PDK1-dependent manner [2]. In viral myocarditis, Bex1 knockout mice showed accelerated coxsackievirus B3-driven heart failure and pathologic heart remodeling, indicating its cardioprotective role during viral infection as it limits viral replication [4].
In conclusion, Bex1 is essential for ciliogenesis and plays significant roles in liver cancer stemness regulation and the cardiac antiviral immune response. Gene knockout mouse models have been key in revealing its functions in these disease areas, helping us understand its biological functions and providing potential therapeutic targets for liver cancer and viral-related heart diseases.
References：
1. Wang, Qian, Liang, Ning, Yang, Tao, He, Xianli, Liu, Chaoxu. 2021. DNMT1-mediated methylation of BEX1 regulates stemness and tumorigenicity in liver cancer. In Journal of hepatology, 75, 1142-1153. doi:10.1016/j.jhep.2021.06.025. https://pubmed.ncbi.nlm.nih.gov/34217777/
2. Wang, Qian, Liang, Ning, Liu, Chaoxu, Yang, Tao, Wang, Gang. 2023. BEX1 supports the stemness of hepatoblastoma by facilitating Warburg effect in a PPARγ/PDK1 dependent manner. In British journal of cancer, 129, 1477-1489. doi:10.1038/s41416-023-02418-4. https://pubmed.ncbi.nlm.nih.gov/37715024/
3. Hibino, Emi, Ichiyama, Yusuke, Tsukamura, Atsushi, Nishimura, Masaki, Mori, Masaki. 2022. Bex1 is essential for ciliogenesis and harbours biomolecular condensate-forming capacity. In BMC biology, 20, 42. doi:10.1186/s12915-022-01246-x. https://pubmed.ncbi.nlm.nih.gov/35144600/
4. Martens, Colton R, Dorn, Lisa E, Kenney, Adam D, Yount, Jacob S, Accornero, Federica. 2022. BEX1 is a critical determinant of viral myocarditis. In PLoS pathogens, 18, e1010342. doi:10.1371/journal.ppat.1010342. https://pubmed.ncbi.nlm.nih.gov/35192678/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen