C57BL/6JCya-Ring1em1flox/Cya
Common Name:
Ring1-flox
Product ID:
S-CKO-04803
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ring1-flox
Strain ID
CKOCMP-19763-Ring1-B6J-VA
Gene Name
Product ID
S-CKO-04803
Gene Alias
Ring1A
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ring1em1flox/Cya mice (Catalog S-CKO-04803) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025183
NCBI RefSeq
NM_009066
Target Region
Exon 3~7
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Ring1, also known as ring finger protein 1, is an essential component of the polycomb-group proteins. It is a catalytic subunit within Polycomb repressive complex 1 (PRC1) along with RNF2, and modifies chromatin through catalyzing histone H2A lysine 119 monoubiquitination (H2AK119ub1). It is involved in various biological processes such as development, cell cycle regulation, and DNA damage repair, and is associated with multiple signaling pathways like Wnt/β-catenin and Notch signaling [1,2,6].
In neurogenesis, hypomorphic RING1 in neural progenitor cells (NPCs) leads to reduced H2AK119ub1, causing delayed DNA damage repair and cell cycle progression, while not significantly impacting NPC differentiation [1]. In the context of forelimb bud development, depletion of RING1A and RING1B (essential PRC1 components) results in severe forelimb formation defects along the proximal-distal (PD) axis due to failure in repressing proximal signal circuitry including Meis2 [3]. In hepatic progenitor cells (HPCs), overexpression of Ring1 promotes their transformation into cancer stem-like cells via the Wnt/β-catenin signaling pathway [2]. In non-small cell lung cancer, high expression of RING1 is associated with poor prognosis, and its knockdown suppresses cell growth through G1/S cell cycle arrest [4]. In breast cancer, low RING1 expression is an unfavorable prognostic factor [5].
In conclusion, Ring1 is crucial for multiple biological processes, including neurogenesis, limb development, and cell differentiation. Its role in diseases such as non-small cell lung cancer and breast cancer, as revealed by functional studies including gene knockout-related models, emphasizes its potential as a biomarker and therapeutic target in cancer research.
References:
1. Ryan, C W, Regan, S L, Mills, E F, Srivastava, A, Bielas, S L. 2024. RING1 missense variants reveal sensitivity of DNA damage repair to H2A monoubiquitination dosage during neurogenesis. In Nature communications, 15, 7931. doi:10.1038/s41467-024-52292-8. https://pubmed.ncbi.nlm.nih.gov/39256363/
2. Zhu, Kai, Li, Jiangwei, Li, Jun, Kong, Guangyao, Li, Zongfang. 2019. Ring1 promotes the transformation of hepatic progenitor cells into cancer stem cells through the Wnt/β-catenin signaling pathway. In Journal of cellular biochemistry, 121, 3941-3951. doi:10.1002/jcb.29496. https://pubmed.ncbi.nlm.nih.gov/31696964/
3. Yakushiji-Kaminatsui, Nayuta, Kondo, Takashi, Endo, Takaho A, Vidal, Miguel, Koseki, Haruhiko. 2015. RING1 proteins contribute to early proximal-distal specification of the forelimb bud by restricting Meis2 expression. In Development (Cambridge, England), 143, 276-85. doi:10.1242/dev.127506. https://pubmed.ncbi.nlm.nih.gov/26674308/
4. Zhou, Yiqun, Wan, Chunhua, Liu, Yifei, Mao, Guoxin, Xue, Qun. 2014. Polycomb group oncogene RING1 is over-expressed in non-small cell lung cancer. In Pathology oncology research : POR, 20, 549-56. doi:10.1007/s12253-013-9727-9. https://pubmed.ncbi.nlm.nih.gov/24414991/
5. Gao, Song, Wang, Si-Yu, Zhang, Xing-Da, Wu, Hao, Pang, Da. 2021. Low Expression of the Polycomb Protein RING1 Predicts Poor Prognosis in Human Breast Cancer. In Frontiers in oncology, 10, 618768. doi:10.3389/fonc.2020.618768. https://pubmed.ncbi.nlm.nih.gov/33634028/
6. Qin, Hongyan, Wang, Jishu, Liang, Yingmin, Tanigaki, Kenji, Han, Hua. 2004. RING1 inhibits transactivation of RBP-J by Notch through interaction with LIM protein KyoT2. In Nucleic acids research, 32, 1492-501. doi:. https://pubmed.ncbi.nlm.nih.gov/14999091/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen