C57BL/6JCya-Ripk1em1flox/Cya
Common Name:
Ripk1-flox
Product ID:
S-CKO-04805
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ripk1-flox
Strain ID
CKOCMP-19766-Ripk1-B6J-VA
Gene Name
Product ID
S-CKO-04805
Gene Alias
D330015H01Rik; RIP; RIP-1; Rinp; Rip1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ripk1em1flox/Cya mice (Catalog S-CKO-04805) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000167374
NCBI RefSeq
NM_009068
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Ripk1, short for receptor-interacting protein kinase 1, is a key mediator of cell death and inflammation. It functions in coordinating cell survival, inflammation, and immunogenic cell death (ICD) [1]. Ripk1 is involved in pathways such as apoptosis, necroptosis, and inflammatory pathways downstream of death receptors (DRs) and pattern recognition receptors (PRRs) [5]. Genetic models, like KO or CKO mouse models, have been crucial in studying its functions.
In mouse models, RIPK1-deficient mice die soon after birth, highlighting its essential role in development [2]. In the context of diseases, genetic and pharmacological inhibition of RIPK1 kinase activity in experimental COPD using Ripk1 S25D/S25D kinase-deficient mice and the RIPK1 kinase inhibitor GSK'547 significantly attenuated airway inflammation, remodelling, emphysema, and apoptotic and necroptotic cell death [3]. In ALS, loss of OPTN led to axonal degeneration through engagement of necroptotic machinery including RIPK1, and RIPK1-and RIPK3-mediated axonal pathology was observed in SOD1(G93A) transgenic mice [4].
In conclusion, Ripk1 is vital in regulating cell death and inflammation processes. Mouse models, especially KO/CKO models, have been instrumental in revealing its role in diseases such as COPD and ALS. Targeting Ripk1 shows promise as a therapeutic approach for these and other related diseases [3,4].
References:
1. Mannion, Jonathan, Gifford, Valentina, Bellenie, Benjamin, Tenev, Tencho, Meier, Pascal. 2024. A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death. In Immunity, 57, 1514-1532.e15. doi:10.1016/j.immuni.2024.04.025. https://pubmed.ncbi.nlm.nih.gov/38788712/
2. Newton, Kim. 2015. RIPK1 and RIPK3: critical regulators of inflammation and cell death. In Trends in cell biology, 25, 347-53. doi:10.1016/j.tcb.2015.01.001. https://pubmed.ncbi.nlm.nih.gov/25662614/
3. Van Eeckhoutte, Hannelore P, Donovan, Chantal, Kim, Richard Y, Hansbro, Philip M, Bracke, Ken R. 2023. RIPK1 kinase-dependent inflammation and cell death contribute to the pathogenesis of COPD. In The European respiratory journal, 61, . doi:10.1183/13993003.01506-2022. https://pubmed.ncbi.nlm.nih.gov/36549711/
4. Ito, Yasushi, Ofengeim, Dimitry, Najafov, Ayaz, Ravits, John, Yuan, Junying. . RIPK1 mediates axonal degeneration by promoting inflammation and necroptosis in ALS. In Science (New York, N.Y.), 353, 603-8. doi:10.1126/science.aaf6803. https://pubmed.ncbi.nlm.nih.gov/27493188/
5. Zhang, Jiahui, Jin, Taijie, Aksentijevich, Ivona, Zhou, Qing. 2021. RIPK1-Associated Inborn Errors of Innate Immunity. In Frontiers in immunology, 12, 676946. doi:10.3389/fimmu.2021.676946. https://pubmed.ncbi.nlm.nih.gov/34163478/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen