C57BL/6JCya-Robo1em1flox/Cya
Common Name:
Robo1-flox
Product ID:
S-CKO-04818
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Robo1-flox
Strain ID
CKOCMP-19876-Robo1-B6J-VA
Gene Name
Product ID
S-CKO-04818
Gene Alias
DUTT1; Gm310
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Robo1em1flox/Cya mice (Catalog S-CKO-04818) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023600
NCBI RefSeq
NM_019413
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Robo1, short for roundabout homolog 1, is a transmembrane receptor. It plays a crucial role in cell growth, migration, and axon guidance, often through interaction with its ligand, Slit proteins. The Slit2-Robo1 signaling pathway is involved in multiple biological processes and diseases [1,2,4,5,6,8]. Genetic models, such as gene knockout (KO) and conditional knockout (CKO) mouse models, have been valuable in studying Robo1's functions.
In liver fibrosis, Robo1 activation promotes liver injury and fibrosis via hepatic stellate cell (HSC) activation. Mice with Slit2 over-expression (Slit2-Tg) were more vulnerable to CCl4-induced liver injury and fibrosis, while Robo1/2(+/-) double heterozygotes showed attenuated liver injury and fibrosis [1]. In pressure-overload-induced cardiac hypertrophy, cardiomyocyte-specific deletion of ROBO1 preserved left ventricular function and abrogated hypertrophy [2]. In glioblastoma, targeting ROBO1 with CAR T cells doubled median survival in recurrent GBM models [3]. In pancreatic ductal adenocarcinoma, a neutralizing antibody against ROBO1 significantly attenuated liver metastasis by disrupting co-adaptation [6]. In epilepsy, Robo1 knockdown in a mouse model prolonged seizure latency and reduced seizure grade, suggesting its role in epileptogenesis [7].
In conclusion, Robo1 is essential in regulating cell growth, migration, and axon guidance. Studies using KO/CKO mouse models have revealed its role in various diseases including liver fibrosis, cardiac hypertrophy, glioblastoma, pancreatic cancer liver metastasis, and epilepsy. These findings provide insights into potential therapeutic strategies targeting Robo1 for these diseases.
References:
1. Chang, Jianlan, Lan, Tian, Li, Changzheng, Ding, Yanqing, Wang, Lijing. 2015. Activation of Slit2-Robo1 signaling promotes liver fibrosis. In Journal of hepatology, 63, 1413-20. doi:10.1016/j.jhep.2015.07.033. https://pubmed.ncbi.nlm.nih.gov/26264936/
2. Liu, Xiaoxiao, Li, Baolei, Wang, Shuyun, Weiss, Stephen, Si, Ming-Sing. 2024. Stromal Cell-SLIT3/Cardiomyocyte-ROBO1 Axis Regulates Pressure Overload-Induced Cardiac Hypertrophy. In Circulation research, 134, 913-930. doi:10.1161/CIRCRESAHA.122.321292. https://pubmed.ncbi.nlm.nih.gov/38414132/
3. Chokshi, Chirayu R, Shaikh, Muhammad Vaseem, Brakel, Benjamin, Moffat, Jason, Singh, Sheila K. 2024. Targeting axonal guidance dependencies in glioblastoma with ROBO1 CAR T cells. In Nature medicine, 30, 2936-2946. doi:10.1038/s41591-024-03138-9. https://pubmed.ncbi.nlm.nih.gov/39095594/
4. Xu, Yun, Li, Wen-Liang, Fu, Li, Gu, Feng, Ma, Yong-Jie. . Slit2/Robo1 signaling in glioma migration and invasion. In Neuroscience bulletin, 26, 474-8. doi:. https://pubmed.ncbi.nlm.nih.gov/21113198/
5. Ahirwar, Dinesh K, Peng, Bo, Charan, Manish, Miles, Wayne O, Ganju, Ramesh K. 2023. Slit2/Robo1 signaling inhibits small-cell lung cancer by targeting β-catenin signaling in tumor cells and macrophages. In Molecular oncology, 17, 839-856. doi:10.1002/1878-0261.13289. https://pubmed.ncbi.nlm.nih.gov/35838343/
6. Li, Qing, Zhang, Xiao-Xin, Hu, Li-Peng, Li, Jun, Zhang, Zhi-Gang. 2023. Coadaptation fostered by the SLIT2-ROBO1 axis facilitates liver metastasis of pancreatic ductal adenocarcinoma. In Nature communications, 14, 861. doi:10.1038/s41467-023-36521-0. https://pubmed.ncbi.nlm.nih.gov/36792623/
7. Liu, Zheng, Huang, Wei, Zhu, Man-Min, Yu, Chang-Yin, Huang, Hao. 2023. Mechanism of Robo1 in the pentylenetetrazol-kindled epilepsy mouse model. In Ibrain, 9, 369-380. doi:10.1002/ibra.12127. https://pubmed.ncbi.nlm.nih.gov/38680506/
8. Bhosle, Vikrant K, Tan, Joel Mj, Li, Taoyingnan, Kim, Peter K, Brumell, John H. 2023. SLIT2/ROBO1 signaling suppresses mTORC1 for organelle control and bacterial killing. In Life science alliance, 6, . doi:10.26508/lsa.202301964. https://pubmed.ncbi.nlm.nih.gov/37311584/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen