C57BL/6JCya-Rpl27em1flox/Cya
Common Name:
Rpl27-flox
Product ID:
S-CKO-04839
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rpl27-flox
Strain ID
CKOCMP-19942-Rpl27-B6J-VA
Gene Name
Product ID
S-CKO-04839
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rpl27em1flox/Cya mice (Catalog S-CKO-04839) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000077856
NCBI RefSeq
NM_011289
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Rpl27, encoding ribosomal protein L27, is involved in pre-rRNA processing and erythropoiesis [4,5]. It is also associated with ribosome complex formation, which is crucial for protein synthesis, a fundamental biological process [3]. Genetic models can be used to further explore its function.
Knockdown of Rpl27 in human colorectal cancer cell lines HCT116 and HT29 suppressed cell proliferation, cell cycle progression, and induced apoptotic cell death. In nude mice, targeting Rpl27 inhibited the growth of human CRC xenografts. This was accompanied by down-regulation of polo-like kinase 1 (PLK1) and G2/M-associated regulators, reducing the migration, invasion, and sphere-forming capacity of CRC cells [1].
In human liver cancer cell lines SNU449 and HepG2, Rpl27 knockdown decreased cell viability, proliferation rate, and increased apoptotic cells, suggesting Rpl27 is important for HCC cell proliferation and apoptosis [2].
In Diamond-Blackfan anemia, mutations in Rpl27 were identified. In vitro knockdown of Rpl27 expression disturbed pre-ribosomal RNA processing, and zebrafish models of rpl27 mutations showed impairments of erythrocyte production [5]. A case of Diamond-Blackfan anemia was associated with a digenic interaction between non-allelic RPS19 and RPL27 variants [6].
In conclusion, Rpl27 is essential for processes like pre-rRNA processing, erythropoiesis, and cell proliferation. Studies using gene knockdown in cell lines and animal models have revealed its significant role in diseases such as colorectal cancer, liver cancer, and Diamond-Blackfan anemia, providing potential therapeutic targets for these diseases.
References:
1. Park, So-Young, Seo, Daekwan, Jeon, Eun-Hye, Cho, Chi Heum, Lee, Yun-Han. 2023. RPL27 contributes to colorectal cancer proliferation and stemness via PLK1 signaling. In International journal of oncology, 63, . doi:10.3892/ijo.2023.5541. https://pubmed.ncbi.nlm.nih.gov/37387446/
2. Suo, Lida, Gao, Mingwei, Ma, Taiheng, Gao, Zhenming. 2023. Effect of RPL27 knockdown on the proliferation and apoptosis of human liver cancer cells. In Biochemical and biophysical research communications, 682, 156-162. doi:10.1016/j.bbrc.2023.10.012. https://pubmed.ncbi.nlm.nih.gov/37812860/
3. Suzuki, Masayoshi, Tezuka, Kenta, Handa, Takumi, Tano, Mitsutoshi, Uchida, Yasuo. 2022. Upregulation of ribosome complexes at the blood-brain barrier in Alzheimer's disease patients. In Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 42, 2134-2150. doi:10.1177/0271678X221111602. https://pubmed.ncbi.nlm.nih.gov/35766008/
4. Glass, Joshua, Weston, Julia, Lewanda, Amy Feldman, Majumdar, Suvankar. 2024. De Novo Variant in the RPL27 Gene in a Second Infant with Diamond-Blackfan Anemia. In Case reports in hematology, 2024, 6626388. doi:10.1155/2024/6626388. https://pubmed.ncbi.nlm.nih.gov/38988374/
5. Wang, RuNan, Yoshida, Kenichi, Toki, Tsutomu, Ogawa, Seishi, Ito, Etsuro. 2014. Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia. In British journal of haematology, 168, 854-64. doi:10.1111/bjh.13229. https://pubmed.ncbi.nlm.nih.gov/25424902/
6. Furuta, Yutaka, Tinker, Rory J, Gulsevin, Alican, Joos, Karen M, Phillips, John A. 2023. Probable digenic inheritance of Diamond-Blackfan anemia. In American journal of medical genetics. Part A, 194, e63454. doi:10.1002/ajmg.a.63454. https://pubmed.ncbi.nlm.nih.gov/37897121/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen