C57BL/6JCya-Sub1em1flox/Cya
Common Name:
Sub1-flox
Product ID:
S-CKO-04853
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sub1-flox
Strain ID
CKOCMP-20024-Sub1-B6J-VA
Gene Name
Product ID
S-CKO-04853
Gene Alias
P15; P9; Pc4; Rpo2tc1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sub1em1flox/Cya mice (Catalog S-CKO-04853) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022816
NCBI RefSeq
NM_011294
Target Region
Exon 3~4
Size of Effective Region
~5.3 kb
Detailed Document
Overview of Gene Research
Sub1, also known as the transcriptional coactivator PC4 in humans, is a DNA-binding protein with multiple functions. It was initially identified as a co-activator during transcription initiation. It is involved in mRNA biogenesis processes such as elongation, termination, and RNAPII phosphorylation, and also participates in RNAPIII transcription. Sub1 has been associated with maintaining genome stability and is involved in DNA-dependent processes like replication and DNA repair [1,3,4].
In atherosclerotic mice models, transgenic myeloid-specific Sub1 knockout in ApoE-/-mice showed that Sub1 mediates the pro-atherosclerotic effects of TLR2 and TLR4 signaling. Sub1 knockout in macrophages enhanced anti-inflammatory M2 macrophage polarization and cholesterol efflux, and irradiated Ldlr-/-mice transplanted with Sub1-/-murine bone marrow displayed reduced atherosclerosis. Promoter analysis revealed Sub1-dependent activation of Irf1 transcription in a Ck2-dependent manner, and Sub1-knockout macrophages had decreased Irf1 expression [5]. In colorectal cancer, depletion of SUB1 in vitro and in vivo inhibited the invasive and metastatic abilities of CRC cells. SUB1 activated NF-κB signaling by modulating UBR5-mediated ubiquitination of UBXN1, driving CRC metastasis [2].
In conclusion, Sub1 is a multifaceted factor involved in transcription and genome stability. Studies using knockout mouse models have revealed its role in diseases like atherosclerosis and colorectal cancer. These models help in understanding how Sub1 contributes to disease-related biological processes, potentially providing new therapeutic targets for these diseases.
References:
1. Calvo, Olga. 2017. Sub1 and RNAPII, until termination does them part. In Transcription, 9, 52-60. doi:10.1080/21541264.2017.1333558. https://pubmed.ncbi.nlm.nih.gov/28853990/
2. Wang, Hao, Chen, Wenwen, Wang, Yanting, Wang, Liangjing, Xue, Meng. 2024. SUB1 promotes colorectal cancer metastasis by activating NF-κB signaling via UBR5-mediated ubiquitination of UBXN1. In Science China. Life sciences, 67, 1199-1211. doi:10.1007/s11427-023-2429-5. https://pubmed.ncbi.nlm.nih.gov/38240906/
3. Garavís, Miguel, Calvo, Olga. 2017. Sub1/PC4, a multifaceted factor: from transcription to genome stability. In Current genetics, 63, 1023-1035. doi:10.1007/s00294-017-0715-6. https://pubmed.ncbi.nlm.nih.gov/28567479/
4. Conesa, Christine, Acker, Joël. 2010. Sub1/PC4 a chromatin associated protein with multiple functions in transcription. In RNA biology, 7, 287-90. doi:. https://pubmed.ncbi.nlm.nih.gov/20305379/
5. Huang, Rongzhong, Hu, Zicheng, Chen, Xiaorui, Du, Jianlin, Chen, Yunqing. 2021. The Transcription Factor SUB1 Is a Master Regulator of the Macrophage TLR Response in Atherosclerosis. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8, e2004162. doi:10.1002/advs.202004162. https://pubmed.ncbi.nlm.nih.gov/34378353/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen