C57BL/6JCya-Rps14em1flox/Cya
Common Name:
Rps14-flox
Product ID:
S-CKO-04856
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rps14-flox
Strain ID
CKOCMP-20044-Rps14-B6J-VA
Gene Name
Product ID
S-CKO-04856
Gene Alias
2600014J02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rps14em1flox/Cya mice (Catalog S-CKO-04856) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025511
NCBI RefSeq
NM_020600
Target Region
Exon 3~4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Rps14, the ribosomal protein S14, is a component of the 40S ribosomal subunit and is crucial for ribosomal biogenesis. It may be involved in the formation of pre-RNA 18s, an intermediate for the 40S small ribosomal subunit. Rps14 is associated with multiple biological processes and signaling pathways, such as p53 and AMPK signaling [2].
Rps14 haploinsufficiency in mouse models recapitulates some features of the 5q-syndrome, like more severe anemia and megakaryocyte morphology changes. Macrophages in these mice show activation of the innate immune response [3]. In glioma, loss-of-function experiments revealed that Rps14 depletion suppresses cell proliferation, migration and promotes apoptosis, with its effects closely related to the p53 signaling pathway [1]. In ER-positive breast cancer cells, downregulation of Rps14 inhibits cell proliferation, cycle, and metastasis, and induces apoptosis [4].
In conclusion, Rps14 plays essential roles in ribosomal biogenesis and is involved in various biological processes and disease conditions. Mouse models with Rps14 haploinsufficiency have been valuable in understanding its role in the 5q-syndrome. Studies in glioma and breast cancer through loss-of-function experiments have further elucidated its role in tumor-related processes, highlighting its potential as a therapeutic target in these diseases.
References:
1. Hu, Shangwei, Cai, Jianhui, Fang, Hua, Zhang, Jicai, Cai, Run. 2022. RPS14 promotes the development and progression of glioma via p53 signaling pathway. In Experimental cell research, 423, 113451. doi:10.1016/j.yexcr.2022.113451. https://pubmed.ncbi.nlm.nih.gov/36535509/
2. Hurtado, Rodrigo, Ramirez, Alexander, Nabipur, Leena, Flores, Josue, Tirado, Carlos A. . The Key Role of the RPS14 Gene in Neoplasms and Solid Tumors. In Journal of the Association of Genetic Technologists, 49, 121-126. doi:. https://pubmed.ncbi.nlm.nih.gov/37665724/
3. Ribezzo, Flavia, Snoeren, Inge A M, Ziegler, Susanne, Ebert, Benjamin L, Schneider, Rebekka K. 2019. Rps14, Csnk1a1 and miRNA145/miRNA146a deficiency cooperate in the clinical phenotype and activation of the innate immune system in the 5q- syndrome. In Leukemia, 33, 1759-1772. doi:10.1038/s41375-018-0350-3. https://pubmed.ncbi.nlm.nih.gov/30651631/
4. Wang, Xiu, Yao, Shuang, Luo, Guanghua, Zhou, Ying, Fang, Qi. . Downregulation of RPS14 inhibits the proliferation and metastasis of estrogen receptor-positive breast cancer cells. In Anti-cancer drugs, 32, 1019-1028. doi:10.1097/CAD.0000000000001112. https://pubmed.ncbi.nlm.nih.gov/34261921/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen