C57BL/6JCya-Rps29em1flox/Cya
Common Name:
Rps29-flox
Product ID:
S-CKO-04862
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rps29-flox
Strain ID
CKOCMP-20090-Rps29-B6J-VA
Gene Name
Product ID
S-CKO-04862
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rps29em1flox/Cya mice (Catalog S-CKO-04862) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037023
NCBI RefSeq
NM_009093
Target Region
Exon 1~2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Rps29, a component of the small 40S ribosomal subunit, is essential for rRNA processing and ribosome biogenesis [2]. It is involved in various biological processes and is associated with multiple pathways, such as those related to hematopoietic development and potentially insecticide resistance [1,3]. Genetic models like zebrafish have been valuable for studying its functions.
In zebrafish, rps29(-/-) embryos exhibit hematopoietic defects including decreased hematopoietic stem cells, red blood cell defects, and increased apoptosis in the head. These effects are mediated by p53, as a p53 mutation nearly completely rescues the rps29-related morphological and hematopoietic phenotypes [1]. In humans, mutations in RPS29 have been identified in families with Diamond-Blackfan anemia, leading to haploinsufficiency of RPS29 expression and defective erythropoiesis [2]. In mosquitoes, RPS29 regulates deltamethrin resistance by binding and degrading CYP6N3, a member of the CYP450 superfamily [3].
In conclusion, Rps29 is crucial for ribosome-related functions and plays significant roles in hematopoietic development and disease, as well as in insecticide resistance mechanisms. Studies using zebrafish models have provided key insights into the role of Rps29 in hematopoietic-related diseases like Diamond-Blackfan anemia, highlighting its importance in understanding these biological processes and disease conditions.
References:
1. Taylor, Alison M, Humphries, Jessica M, White, Richard M, Burns, Caroline E, Zon, Leonard I. 2011. Hematopoietic defects in rps29 mutant zebrafish depend upon p53 activation. In Experimental hematology, 40, 228-237.e5. doi:. https://pubmed.ncbi.nlm.nih.gov/22120640/
2. Mirabello, Lisa, Macari, Elizabeth R, Jessop, Lea, Zon, Leonard, Savage, Sharon A. 2014. Whole-exome sequencing and functional studies identify RPS29 as a novel gene mutated in multicase Diamond-Blackfan anemia families. In Blood, 124, 24-32. doi:10.1182/blood-2013-11-540278. https://pubmed.ncbi.nlm.nih.gov/24829207/
3. Yu, Jing, Hu, Shengli, Ma, Kai, Shen, Bo, Zhu, Changliang. 2014. Ribosomal protein S29 regulates metabolic insecticide resistance through binding and degradation of CYP6N3. In PloS one, 9, e94611. doi:10.1371/journal.pone.0094611. https://pubmed.ncbi.nlm.nih.gov/24728095/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen