C57BL/6JCya-S100a10em1flox/Cya
Common Name:
S100a10-flox
Product ID:
S-CKO-04896
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
S100a10-flox
Strain ID
CKOCMP-20194-S100a10-B6J-VA
Gene Name
Product ID
S-CKO-04896
Gene Alias
42C; CAL12; CLP11; Cal1l; p10; p11
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-S100a10em1flox/Cya mice (Catalog S-CKO-04896) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045756
NCBI RefSeq
NM_009112
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
S100A10, also known as p11, is a multifunctional protein belonging to the S100 family of Ca2+-binding proteins, though it itself does not bind Ca2+ [8]. It commonly forms a heterotetrameric complex with Annexin A2, which is essential for the activation of the plasminogen activation pathway, leading to the generation of plasmin from plasminogen. This pathway is involved in various physiological processes such as fibrinolysis, angiogenesis, wound healing, as well as pathological processes like tumor progression [2,3,4,5,6,7].
In cancer research, S100A10 has been found to play significant roles. In hepatocellular carcinoma (HCC), S100A10 promotes HCC initiation, self-renewal, chemoresistance, and metastasis both in vitro and in vivo. It is secreted by HCC cells into extracellular vesicles (EVs), and these S100A10-enriched EVs enhance the stemness and metastatic ability of HCC cells, upregulate certain signaling pathways, and promote epithelial-mesenchymal transition. Blockage of EV-S100A10 with a neutralizing antibody significantly abrogates these enhancing effects [1]. In lung cancer metastasis research, S100A10-deficient mice showed reduced cancer metastasis in the lung. Activation of S100A10 within lung fibroblasts via tumor-derived exosomes increased the expression of certain chemokines, leading to the recruitment of myeloid-derived suppressor cells (MDSCs), and S100A10 inhibitors could block lung metastasis in vivo [9].
In conclusion, S100A10 is crucial in the plasminogen activation pathway and has a significant impact on cancer-related processes. Studies using gene-knockout models, such as S100A10-deficient mice, have revealed its role in cancer metastasis and the regulation of the immune microenvironment, providing potential therapeutic targets for cancer treatment, especially in HCC and lung cancer metastasis [1,9].
References:
1. Wang, Xia, Huang, Hongyang, Sze, Karen Man-Fong, Guan, Xinyuan, Ng, Irene Oi-Lin. 2023. S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos. In Gut, 72, 1370-1384. doi:10.1136/gutjnl-2022-327998. https://pubmed.ncbi.nlm.nih.gov/36631249/
2. Bharadwaj, Alamelu G, Kempster, Emma, Waisman, David M. 2021. The ANXA2/S100A10 Complex-Regulation of the Oncogenic Plasminogen Receptor. In Biomolecules, 11, . doi:10.3390/biom11121772. https://pubmed.ncbi.nlm.nih.gov/34944416/
3. Bharadwaj, Alamelu, Kempster, Emma, Waisman, David Morton. 2021. The Annexin A2/S100A10 Complex: The Mutualistic Symbiosis of Two Distinct Proteins. In Biomolecules, 11, . doi:10.3390/biom11121849. https://pubmed.ncbi.nlm.nih.gov/34944495/
4. Tantyo, Normastuti Adhini, Karyadi, Azrina Saraswati, Rasman, Siti Zulimas, Devina, Astrella, Sumarpo, Anton. 2018. The prognostic value of S100A10 expression in cancer. In Oncology letters, 17, 1417-1424. doi:10.3892/ol.2018.9751. https://pubmed.ncbi.nlm.nih.gov/30675195/
5. Saiki, Yuriko, Horii, Akira. 2019. Multiple functions of S100A10, an important cancer promoter. In Pathology international, 69, 629-636. doi:10.1111/pin.12861. https://pubmed.ncbi.nlm.nih.gov/31612598/
6. Noye, Tannith M, Lokman, Noor A, Oehler, Martin K, Ricciardelli, Carmela. 2018. S100A10 and Cancer Hallmarks: Structure, Functions, and its Emerging Role in Ovarian Cancer. In International journal of molecular sciences, 19, . doi:10.3390/ijms19124122. https://pubmed.ncbi.nlm.nih.gov/30572596/
7. Kwon, Mijung, MacLeod, Travis J, Zhang, Yi, Waisman, David Morton. 2005. S100A10, annexin A2, and annexin a2 heterotetramer as candidate plasminogen receptors. In Frontiers in bioscience : a journal and virtual library, 10, 300-25. doi:. https://pubmed.ncbi.nlm.nih.gov/15574370/
8. Okura, Gillian C, Bharadwaj, Alamelu G, Waisman, David M. 2023. Recent Advances in Molecular and Cellular Functions of S100A10. In Biomolecules, 13, . doi:10.3390/biom13101450. https://pubmed.ncbi.nlm.nih.gov/37892132/
9. Li, Juan, Zhou, Can, Gao, Xiaoqian, Liu, Jie, Liu, Peijun. 2024. S100A10 promotes cancer metastasis via recruitment of MDSCs within the lungs. In Oncoimmunology, 13, 2381803. doi:10.1080/2162402X.2024.2381803. https://pubmed.ncbi.nlm.nih.gov/39071160/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen