C57BL/6JCya-S100a9em1flox/Cya
Common Name:
S100a9-flox
Product ID:
S-CKO-04902
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
S100a9-flox
Strain ID
CKOCMP-20202-S100a9-B6J-VA
Gene Name
Product ID
S-CKO-04902
Gene Alias
60B8Ag; BEE22; Cagb; GAGB; L1Ag; MRP14; p14
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-S100a9em1flox/Cya mice (Catalog S-CKO-04902) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000117167
NCBI RefSeq
NM_009114
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
S100a9, a Ca2+ binding protein, belongs to the S100 family. It is mainly expressed in neutrophils and monocytes and plays crucial roles in modulating inflammatory responses and inflammation-associated diseases. S100a9 often forms a heterodimer with S100a8 (S100A8/A9), participating in multiple signaling pathways like Toll-like receptor 4/MyD88/NF-κB, IL-17-NFκB-caspase-3, and is involved in various biological processes including tumor development, myocardial infarction response, and skin inflammation regulation [1,2,4].
In gene knockout studies, S100a9-KO mice have shown that long-term S100a9 blockade negatively impacts cardiac recovery after myocardial infarction, as it reduces the proliferation of certain hematopoietic stem and progenitor cells and impairs monocyte-macrophage transition [2]. In hepatocellular carcinoma, depletion of S100a9 dampens tumor growth and metastatic ability [3]. In rosacea-like mouse models, inhibition of S100a9 suppresses disease progression and inflammatory responses [4]. In myocardial ischemia-reperfusion mouse models, targeting S100a9 with a specific inhibitor mitigates acute inflammatory damage and fibrosis [5]. In sepsis-induced acute lung injury, S100a9 knockout alleviates lung damage and epithelial cell apoptosis [6]. In sepsis-induced acute liver injury, S100a9-KO mice show attenuated liver dysfunction and injury with improved mitochondrial function [7]. In acute pancreatitis, pancreatic ductal deletion of S100a9 alleviates the injury by inhibiting NLRP3 activation [8].
In conclusion, S100a9 is significantly involved in inflammation, tissue repair, and tumor-related processes. Gene knockout models of S100a9 have revealed its roles in diseases such as myocardial infarction, cancer, rosacea, and various inflammatory diseases, highlighting its potential as a therapeutic target for these conditions.
References:
1. Chen, Yu, Ouyang, Yuzhen, Li, Zhixin, Wang, Xiufang, Ma, Jian. 2023. S100A8 and S100A9 in Cancer. In Biochimica et biophysica acta. Reviews on cancer, 1878, 188891. doi:10.1016/j.bbcan.2023.188891. https://pubmed.ncbi.nlm.nih.gov/37001615/
2. Marinković, Goran, Koenis, Duco Steven, de Camp, Lisa, Jovinge, Stefan, Schiopu, Alexandru. 2020. S100A9 Links Inflammation and Repair in Myocardial Infarction. In Circulation research, 127, 664-676. doi:10.1161/CIRCRESAHA.120.315865. https://pubmed.ncbi.nlm.nih.gov/32434457/
3. Zhong, Chengrui, Niu, Yi, Liu, Wenwu, Yuan, Yunfei, Li, Binkui. 2022. S100A9 Derived from Chemoembolization-Induced Hypoxia Governs Mitochondrial Function in Hepatocellular Carcinoma Progression. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9, e2202206. doi:10.1002/advs.202202206. https://pubmed.ncbi.nlm.nih.gov/36041055/
4. Le, Yan, Zhang, Jiawen, Lin, Yi, Xiang, Leihong, Zhang, Chengfeng. 2024. S100A9 Exacerbates the Inflammation in Rosacea through Toll-Like Receptor 4/MyD88/NF-κB Signaling Pathway. In The Journal of investigative dermatology, 144, 1985-1993.e1. doi:10.1016/j.jid.2024.02.012. https://pubmed.ncbi.nlm.nih.gov/38447867/
5. Shen, Shichun, Zhang, Meng, Wang, Xiaohe, Gong, Chen, Ma, Likun. 2024. Single-cell RNA sequencing reveals S100a9hi macrophages promote the transition from acute inflammation to fibrotic remodeling after myocardial ischemia‒reperfusion. In Theranostics, 14, 1241-1259. doi:10.7150/thno.91180. https://pubmed.ncbi.nlm.nih.gov/38323308/
6. Pei, Hui, Chen, Jianming, Qu, Jie, Lu, Zhongqiu. 2024. S100A9 exacerbates sepsis-induced acute lung injury via the IL17-NFκB-caspase-3 signaling pathway. In Biochemical and biophysical research communications, 710, 149832. doi:10.1016/j.bbrc.2024.149832. https://pubmed.ncbi.nlm.nih.gov/38588614/
7. Zhang, Yanting, Wu, Feng, Teng, Fei, Guo, Shubin, Li, Huihua. 2023. Deficiency of S100A9 Alleviates Sepsis-Induced Acute Liver Injury through Regulating AKT-AMPK-Dependent Mitochondrial Energy Metabolism. In International journal of molecular sciences, 24, . doi:10.3390/ijms24032112. https://pubmed.ncbi.nlm.nih.gov/36768433/
8. Xiang, Hong, Guo, Fangyue, Tao, Xufeng, Li, Lunxu, Shang, Dong. 2021. Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation. In Theranostics, 11, 4467-4482. doi:10.7150/thno.54245. https://pubmed.ncbi.nlm.nih.gov/33754072/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen