C57BL/6JCya-Khdrbs1em1flox/Cya
Common Name:
Khdrbs1-flox
Product ID:
S-CKO-04911
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Khdrbs1-flox
Strain ID
CKOCMP-20218-Khdrbs1-B6J-VA
Gene Name
Product ID
S-CKO-04911
Gene Alias
Sam68; p62; p68
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Khdrbs1em1flox/Cya mice (Catalog S-CKO-04911) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066257
NCBI RefSeq
NM_011317
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
KHDRBS1, also known as Sam68, is an RNA-binding protein that plays crucial roles in multiple biological processes. It is involved in signal transduction and is associated with pathways such as NF-κB activation, which is important for cellular responses to DNA damage [3,5]. It also has a connection with the regulation of RNA stability and gene expression, influencing various biological functions [1,2]. Genetic models, like those in zebrafish, have been valuable in studying its functions [4].
In a zebrafish model of severe liver injury, khdrbs1 was found to be specifically expressed in bipotential progenitor cells (BPPCs). Loss-of-function experiments in zebrafish, using a CRISPR/dead Cas9-mediated system to interfere with khdrbs1 in biliary epithelial cells, led to defective liver regeneration and impaired re-differentiation of BPPCs. The khdrbs1-/-mutant showed impaired proliferation and re-differentiation of BPPCs, and this was associated with the activation of p53 signaling, as tp53 mutation could partially rescue the defective liver regeneration of the khdrbs1-/-mutant [4]. In mouse models, Sam68/KHDRBS1 deficiency abolished DNA damage-stimulated polymers of ADP-ribose (PAR) production and the PAR-dependent NF-κB transactivation of anti-apoptotic genes. Sam68 deleted cells were hypersensitive to genotoxicity, and knockdown of Sam68 sensitized human colon cancer cells to genotoxic stress-induced apoptosis, while genetic deletion of Sam68 dampened colon tumor burden in mice, indicating its role in colon tumorigenesis [5].
In conclusion, KHDRBS1 is essential for processes like liver regeneration in zebrafish and plays a significant role in colon tumorigenesis in mice. Its functions are mainly related to the regulation of cell-related processes such as re-differentiation, proliferation, and responses to genotoxic stress through its influence on signaling pathways like p53 and NF-κB. The use of gene-knockout models in zebrafish and mice has provided crucial insights into its role in these specific biological and disease-related processes.
References:
1. Wong, Tin-Lok, Loh, Jia-Jian, Lu, Shixun, Leung, Suet Yi, Ma, Stephanie. 2023. ADAR1-mediated RNA editing of SCD1 drives drug resistance and self-renewal in gastric cancer. In Nature communications, 14, 2861. doi:10.1038/s41467-023-38581-8. https://pubmed.ncbi.nlm.nih.gov/37208334/
2. Yu, Xin, Kang, Weibiao, Zhang, Jiajia, Chen, Changyu, Liu, Yi. 2022. Shortening of the KHDRBS1 3'UTR by alternative cleavage and polyadenylation alters miRNA-mediated regulation and promotes gastric cancer progression. In American journal of translational research, 14, 6574-6585. doi:. https://pubmed.ncbi.nlm.nih.gov/36247240/
3. Fu, Kai, Sun, Xin, Wier, Eric M, Hobbs, Ryan P, Wan, Fengyi. 2016. Sam68/KHDRBS1-dependent NF-κB activation confers radioprotection to the colon epithelium in γ-irradiated mice. In eLife, 5, . doi:10.7554/eLife.21957. https://pubmed.ncbi.nlm.nih.gov/27996939/
4. Gang, Kai, Chen, Qi, Sun, Junhui, Ni, Rui, Ma, Jianlong. 2025. Khdrbs1 drives re-differentiation of bipotential progenitor cells by inhibiting p53 in zebrafish biliary-mediated liver regeneration. In Development (Cambridge, England), 152, . doi:10.1242/dev.204266. https://pubmed.ncbi.nlm.nih.gov/39963927/
5. Fu, Kai, Sun, Xin, Wier, Eric M, Sears, Cynthia L, Wan, Fengyi. 2016. Sam68/KHDRBS1 is critical for colon tumorigenesis by regulating genotoxic stress-induced NF-κB activation. In eLife, 5, . doi:10.7554/eLife.15018. https://pubmed.ncbi.nlm.nih.gov/27458801/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen