C57BL/6JCya-Sgk1em1flox/Cya
Common Name:
Sgk1-flox
Product ID:
S-CKO-05020
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sgk1-flox
Strain ID
CKOCMP-20393-Sgk1-B6J-VA
Gene Name
Product ID
S-CKO-05020
Gene Alias
Sgk
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sgk1em1flox/Cya mice (Catalog S-CKO-05020) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000120509
NCBI RefSeq
NM_001161845
Target Region
Exon 5~11
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
SGK1, also known as serum and glucocorticoid-induced kinase-1, belongs to the AGC protein kinase family. It phosphorylates serine and threonine residues of target proteins, regulating numerous ion channels and transporters, and promoting cell survival under stress. SGK1 tightly regulates multiple signal transduction pathways, which are related to various biological processes and diseases [1,4].
Genetic deficiency of Sgk1 in mice significantly reduced phosphate-induced calcification and osteo-/chondrogenic transdifferentiation of vascular smooth muscle cells (VSMCs), and aortic calcification, stiffness, and osteo-/chondrogenic transdifferentiation in mice following cholecalciferol overload were strongly reduced by genetic knockout or pharmacological inhibition of Sgk1. Also, Sgk1 deficiency blunted vascular calcification in apolipoprotein E-deficient mice after subtotal nephrectomy, indicating SGK1 as a key regulator of vascular calcification, at least partly, via NF-κB activation [2]. In the context of liver ischemia-reperfusion in colorectal liver metastasis mouse models, administration of the SGK1 inhibitor GSK-650394 reduced ERK-related neutrophil extracellular traps formation and polymorphonuclear myeloid-derived suppressor cell infiltration, alleviating recurrence of liver metastasis [3]. In mice with high-fat diet-induced lung fibrosis, treatment with the SGK1 specific inhibitor EMD638683 alleviated pulmonary fibrosis and activation of the integrin-inflammasome pathway [5].
In conclusion, SGK1 plays essential roles in multiple biological processes. Through gene knockout or inhibitor-based studies in mouse models, its key regulatory functions in vascular calcification, recurrence of liver metastasis, and lung fibrosis have been revealed. These findings provide important insights into the mechanisms of related diseases and potential therapeutic targets.
References:
1. Ghani, Madiha Javeed. 2021. SGK1, autophagy and cancer: an overview. In Molecular biology reports, 49, 675-685. doi:10.1007/s11033-021-06836-6. https://pubmed.ncbi.nlm.nih.gov/34669124/
2. Voelkl, Jakob, Luong, Trang Td, Tuffaha, Rashad, Lang, Florian, Alesutan, Ioana. 2018. SGK1 induces vascular smooth muscle cell calcification through NF-κB signaling. In The Journal of clinical investigation, 128, 3024-3040. doi:10.1172/JCI96477. https://pubmed.ncbi.nlm.nih.gov/29889103/
3. Li, Xiangdong, Wang, Ziyi, Jiao, Chenyu, Han, Sheng, Pu, Liyong. 2023. Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3. In Journal of translational medicine, 21, 121. doi:10.1186/s12967-023-03977-z. https://pubmed.ncbi.nlm.nih.gov/36788538/
4. Sang, Yiwen, Kong, Piaoping, Zhang, Shizhen, Tao, Zhihua, Liu, Weiwei. 2021. SGK1 in Human Cancer: Emerging Roles and Mechanisms. In Frontiers in oncology, 10, 608722. doi:10.3389/fonc.2020.608722. https://pubmed.ncbi.nlm.nih.gov/33542904/
5. Gu, Xin, Meng, Haoyu, Peng, Chengyi, Jin, Jianliang, Wang, Xiaoyan. . Inflammasome activation and metabolic remodelling in p16-positive aging cells aggravates high-fat diet-induced lung fibrosis by inhibiting NEDD4L-mediated K48-polyubiquitin-dependent degradation of SGK1. In Clinical and translational medicine, 13, e1308. doi:10.1002/ctm2.1308. https://pubmed.ncbi.nlm.nih.gov/37345264/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen