C57BL/6JCya-Tra2bem1flox/Cya
Common Name:
Tra2b-flox
Product ID:
S-CKO-05059
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tra2b-flox
Strain ID
CKOCMP-20462-Tra2b-B6J-VA
Gene Name
Product ID
S-CKO-05059
Gene Alias
5730405G21Rik; D16Ertd266e; SIG-41; Sfrs10; Silg41; TRA2beta
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tra2bem1flox/Cya mice (Catalog S-CKO-05059) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000161286
NCBI RefSeq
NM_009186
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Tra2b, also known as SFRS10, is an RNA binding protein that plays well-established roles in developmentally regulated alternative splicing, especially during Drosophila sexual differentiation. In mammals, it is essential for embryogenesis. Precise regulation of alternative splicing by Tra2b is critical to the development and function of the central nervous system [2].
In murine cortical neurogenesis, TRA2B depletion in cortex-specific Tra2b mutant mice (a form of in vivo KO model) results in apoptosis of neural progenitor cells and disorganization of the cortical plate, indicating its essential role in proper cerebral cortex development [2]. In T-cell fate regulation, an evolutionarily ultraconserved poison exon (PE) in Tra2β undergoes autoregulated splicing in response to antigen-receptor stimulation, which is crucial for TCR-induced effector T cell expansion, function, and survival [3]. In endometrial carcinoma, overexpression of TRA2B promotes cell viability, proliferation, invasion, and inhibits apoptosis, while siRNA-TRA2B treatment has the opposite effects, suggesting its role in the pathogenesis of endometrial carcinoma [1]. Also, predicted loss-of-function variants in the 5' portion of TRA2B in humans cause a new neurodevelopmental syndrome through a dominant-negative mechanism involving alternative translation start site usage and overexpression of a shorter Tra2β protein [4].
In conclusion, Tra2b is essential for multiple biological processes such as neural development, T-cell fate determination, and is involved in diseases like endometrial carcinoma and a novel neurodevelopmental syndrome. The use of KO/CKO mouse models and functional studies in human cells have significantly contributed to understanding Tra2b's functions and its implications in disease, highlighting its importance as a potential therapeutic target in related disease areas.
References:
1. Paudel, Dhruba, Ouyang, Yiqin, Huang, Qingting, Dhakal, Bipin, Tong, Xiaowen. 2019. Expression of TRA2B in endometrial carcinoma and its regulatory roles in endometrial carcinoma cells. In Oncology letters, 18, 2455-2463. doi:10.3892/ol.2019.10553. https://pubmed.ncbi.nlm.nih.gov/31452736/
2. Roberts, Jacqueline M, Ennajdaoui, Hanane, Edmondson, Carina, Sanford, Jeremy R, Chen, Bin. . Splicing factor TRA2B is required for neural progenitor survival. In The Journal of comparative neurology, 522, 372-92. doi:10.1002/cne.23405. https://pubmed.ncbi.nlm.nih.gov/23818142/
3. Karginov, Timofey A, Ménoret, Antoine, Leclair, Nathan K, Zhou, Beiyan, Vella, Anthony T. 2024. Autoregulated splicing of TRA2β programs T cell fate in response to antigen-receptor stimulation. In Science (New York, N.Y.), 385, eadj1979. doi:10.1126/science.adj1979. https://pubmed.ncbi.nlm.nih.gov/39265028/
4. Ramond, Francis, Dalgliesh, Caroline, Grimmel, Mona, Haack, Tobias, Elliott, David J. 2022. Clustered variants in the 5' coding region of TRA2B cause a distinctive neurodevelopmental syndrome. In Genetics in medicine : official journal of the American College of Medical Genetics, 25, 100003. doi:10.1016/j.gim.2022.100003. https://pubmed.ncbi.nlm.nih.gov/36549593/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen