C57BL/6JCya-Slc16a1em1flox/Cya
Common Name:
Slc16a1-flox
Product ID:
S-CKO-05084
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Slc16a1-flox
Strain ID
CKOCMP-20501-Slc16a1-B6J-VA
Gene Name
Product ID
S-CKO-05084
Gene Alias
Mct1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc16a1em1flox/Cya mice (Catalog S-CKO-05084) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046212
NCBI RefSeq
NM_009196
Target Region
Exon 3~4
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Slc16a1, also known as monocarboxylate transporter 1 (MCT1), is a proton-dependent transporter that serves as a lactate transporter [1,2]. It is involved in multiple biological processes, and its function is crucial for maintaining normal physiological conditions. It participates in regulating the internal and external environment, glycolysis, and redox homeostasis in cells [4]. Mutations in SLC16A1 have been associated with congenital hyperinsulinism, as it is one of the key genes involved in the regulation of insulin secretion from pancreatic β-cells [3].
In hepatocellular carcinoma (HCC), HCC exosomal SLC16A1-AS1 (antisense RNA of SLC16A1) enhances mRNA stabilization of SLC16A1 in macrophages by promoting the interaction between 3' untranslated regions (3'UTR) of SLC16A1 mRNA and heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1). SLC16A1 then accelerates lactate influx and activates c-Raf/ERK signaling to induce M2 polarization of macrophages. Reciprocally, M2 macrophages secrete IL-6 to promote HCC cell proliferation, invasion, and glycolysis [1]. In cervical cancer, SLC16A1 is highly expressed and is a critical hub for regulating the internal and external environment, glycolysis, and redox homeostasis. Inhibiting SLC16A1 provides a new approach to eliminate cervical cancer [4].
In conclusion, SLC16A1 plays essential roles in multiple biological processes and is closely associated with diseases such as congenital hyperinsulinism, hepatocellular carcinoma, and cervical cancer. Functional studies, especially those using gene knockout or conditional knockout mouse models, could further reveal its detailed functions and underlying mechanisms in these diseases, providing potential therapeutic targets for related disorders [1,3,4].
References:
1. Hu, Yuhang, Li, Yang, Xiong, Hewei, Lv, Guozheng, Zhao, Gang. 2024. Exosomal SLC16A1-AS1-induced M2 macrophages polarization facilitates hepatocellular carcinoma progression. In International journal of biological sciences, 20, 4341-4363. doi:10.7150/ijbs.94440. https://pubmed.ncbi.nlm.nih.gov/39247822/
2. Felmlee, Melanie A, Morse, Bridget L, Morris, Marilyn E. 2021. γ-Hydroxybutyric Acid: Pharmacokinetics, Pharmacodynamics, and Toxicology. In The AAPS journal, 23, 22. doi:10.1208/s12248-020-00543-z. https://pubmed.ncbi.nlm.nih.gov/33417072/
3. Demirbilek, Hüseyin, Hussain, Khalid. 2017. Congenital Hyperinsulinism: Diagnosis and Treatment Update. In Journal of clinical research in pediatric endocrinology, 9, 69-87. doi:10.4274/jcrpe.2017.S007. https://pubmed.ncbi.nlm.nih.gov/29280746/
4. You, Shiwan, Zhang, Jing, Yu, Lan, Akram, Zubair, Sun, Shiguo. 2023. Construction of SLC16A1/3 Targeted Gallic Acid-Iron-Embelin Nanoparticles for Regulating Glycolysis and Redox Pathways in Cervical Cancer. In Molecular pharmaceutics, 20, 4574-4586. doi:10.1021/acs.molpharmaceut.3c00294. https://pubmed.ncbi.nlm.nih.gov/37307591/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen