C57BL/6JCya-Slc22a5em1flox/Cya
Common Name:
Slc22a5-flox
Product ID:
S-CKO-05099
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc22a5-flox
Strain ID
CKOCMP-20520-Slc22a5-B6J-VA
Gene Name
Product ID
S-CKO-05099
Gene Alias
Lstpl; Octn2; jvs
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc22a5em1flox/Cya mice (Catalog S-CKO-05099) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019044
NCBI RefSeq
NM_011396
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Slc22a5, also known as organic cation transporter novel family member 2 (OCTN2), is a crucial gene encoding a transporter essential for carnitine uptake. Carnitine is vital for the β -oxidation of fatty acids, which involves transporting acyl moieties to mitochondria via the carnitine shuttle [1,3,5,6]. This process is integral to cell metabolism, providing an alternative energy source to glucose, especially in cancer cells where its expression can impact growth and proliferation [1].
In a study with the long-chain acyl-CoA dehydrogenase (LCAD) KO mouse, introducing one defective Slc22a5 allele (jvs) decreased free carnitine levels in the liver, kidney, and heart. However, major phenotypes of the LCAD KO mouse such as cardiac hypertrophy, fasting-induced hypoglycemia, and increased liver weight were not affected, suggesting that secondary carnitine deficiency may not play a major role in the pathophysiology of these phenotypes [4]. In macrophages, conditional knockout (CKO) of SIRPA transcriptionally downregulates Slc22a5, and targeting Slc22a5 renders mitophagy inhibition of macrophages in response to LPS stimuli, improving survival and deterring the development of septic acute kidney injury [2].
In conclusion, Slc22a5 is essential for maintaining carnitine homeostasis, which is crucial for fatty acid oxidation and cell metabolism. Model-based research, especially the use of KO and CKO mouse models, has provided insights into its role in various biological processes and disease conditions such as septic acute kidney injury and the pathophysiology related to fatty acid oxidation disorders [2,4].
References:
1. Juraszek, Barbara, Nałęcz, Katarzyna A. 2019. SLC22A5 (OCTN2) Carnitine Transporter-Indispensable for Cell Metabolism, a Jekyll and Hyde of Human Cancer. In Molecules (Basel, Switzerland), 25, . doi:10.3390/molecules25010014. https://pubmed.ncbi.nlm.nih.gov/31861504/
2. Jia, Yu, Li, Jun-Hua, Hu, Bang-Chuan, Ye, Xiang-Ming, Mo, Shi-Jing. 2024. Targeting SLC22A5 fosters mitophagy inhibition-mediated macrophage immunity against septic acute kidney injury upon CD47-SIRPα axis blockade. In Heliyon, 10, e26791. doi:10.1016/j.heliyon.2024.e26791. https://pubmed.ncbi.nlm.nih.gov/38586373/
3. Longo, Nicola, Frigeni, Marta, Pasquali, Marzia. 2016. Carnitine transport and fatty acid oxidation. In Biochimica et biophysica acta, 1863, 2422-35. doi:10.1016/j.bbamcr.2016.01.023. https://pubmed.ncbi.nlm.nih.gov/26828774/
4. Ranea-Robles, Pablo, Yu, Chunli, van Vlies, Naomi, Vaz, Frédéric M, Houten, Sander M. 2019. Slc22a5 haploinsufficiency does not aggravate the phenotype of the long-chain acyl-CoA dehydrogenase KO mouse. In Journal of inherited metabolic disease, 43, 486-495. doi:10.1002/jimd.12204. https://pubmed.ncbi.nlm.nih.gov/31845336/
5. Kou, Longfa, Sun, Rui, Ganapathy, Vadivel, Yao, Qing, Chen, Ruijie. 2018. Recent advances in drug delivery via the organic cation/carnitine transporter 2 (OCTN2/SLC22A5). In Expert opinion on therapeutic targets, 22, 715-726. doi:10.1080/14728222.2018.1502273. https://pubmed.ncbi.nlm.nih.gov/30016594/
6. Tamai, Ikumi. 2012. Pharmacological and pathophysiological roles of carnitine/organic cation transporters (OCTNs: SLC22A4, SLC22A5 and Slc22a21). In Biopharmaceutics & drug disposition, 34, 29-44. doi:10.1002/bdd.1816. https://pubmed.ncbi.nlm.nih.gov/22952014/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen