C57BL/6JCya-Slc2a1em1flox/Cya
Common Name:
Slc2a1-flox
Product ID:
S-CKO-05104
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Slc2a1-flox
Strain ID
CKOCMP-20525-Slc2a1-B6J-VA
Gene Name
Product ID
S-CKO-05104
Gene Alias
GT1; Glut-1; Glut1; M100200; Rgsc200
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc2a1em1flox/Cya mice (Catalog S-CKO-05104) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030398
NCBI RefSeq
NM_011400
Target Region
Exon 3~10
Size of Effective Region
~4.6 kb
Detailed Document
Overview of Gene Research
Slc2a1, also known as solute carrier family 2 member 1, encodes glucose transporter-1 (GLUT1), which is critical in the metabolism of glucose, an energy source for cell growth. It is involved in the glycolysis metabolism pathway, and plays an important role in maintaining normal cell function and energy supply [1,2,3,4].
In lung squamous cell carcinoma (LUSC), SLC2A1 was found to be differentially expressed between tumor and normal tissues. High expression of SLC2A1 was associated with worse survival and poor pathological response to adjuvant immunochemotherapy, and it promoted macrophage polarization into the M2 phenotype [1].
In hepatocellular carcinoma (HCC), isoginkgetin (ISO) suppressed SLC2A1/GLUT1 expression and glucose uptake, leading to activation of the AMPK-ULK1 axis and autophagy [2].
In gastric cancer, high SLC2A1 expression was associated with poor prognosis, cancer cell proliferation, decreased immune cells (including CD8 T cells and B cells), and was indirectly linked to the retinoic signaling pathway and negatively regulated immune cells/receptors [3].
In pancreatic cancer, FOXD1 promoted SLC2A1 transcription and inhibited its degradation, enhancing GLUT1 expression and facilitating cancer cell proliferation, invasion, and metastasis through regulating aerobic glycolysis [4].
In conclusion, Slc2a1 is crucial for glucose metabolism and is implicated in multiple diseases, especially various cancers. Studies on Slc2a1 help to understand the role of glucose metabolism in disease development, providing potential biomarkers for diagnosis and new targets for treatment.
References：
1. Hao, Bo, Dong, Huixing, Xiong, Rui, Li, Ning, Geng, Qing. 2024. Identification of SLC2A1 as a predictive biomarker for survival and response to immunotherapy in lung squamous cell carcinoma. In Computers in biology and medicine, 171, 108183. doi:10.1016/j.compbiomed.2024.108183. https://pubmed.ncbi.nlm.nih.gov/38422959/
2. Yao, Jie, Tang, Shuming, Shi, Chenyan, Tian, Jun, Zeng, Xiaobin. 2022. Isoginkgetin, a potential CDK6 inhibitor, suppresses SLC2A1/GLUT1 enhancer activity to induce AMPK-ULK1-mediated cytotoxic autophagy in hepatocellular carcinoma. In Autophagy, 19, 1221-1238. doi:10.1080/15548627.2022.2119353. https://pubmed.ncbi.nlm.nih.gov/36048765/
3. Min, Kyueng-Whan, Kim, Dong-Hoon, Son, Byoung Kwan, Koh, Young Wha, Oh, Il Hwan. 2021. High SLC2A1 expression associated with suppressing CD8 T cells and B cells promoted cancer survival in gastric cancer. In PloS one, 16, e0245075. doi:10.1371/journal.pone.0245075. https://pubmed.ncbi.nlm.nih.gov/33735188/
4. Cai, Kun, Chen, Shiyu, Zhu, Changhao, He, Zhiwei, Sun, Chengyi. 2022. FOXD1 facilitates pancreatic cancer cell proliferation, invasion, and metastasis by regulating GLUT1-mediated aerobic glycolysis. In Cell death & disease, 13, 765. doi:10.1038/s41419-022-05213-w. https://pubmed.ncbi.nlm.nih.gov/36057597/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen