C57BL/6JCya-Slc2a2em1flox/Cya
Common Name:
Slc2a2-flox
Product ID:
S-CKO-05105
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Slc2a2-flox
Strain ID
CKOCMP-20526-Slc2a2-B6J-VA
Gene Name
Product ID
S-CKO-05105
Gene Alias
Glut-2; Glut2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc2a2em1flox/Cya mice (Catalog S-CKO-05105) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029240
NCBI RefSeq
NM_031197
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Slc2a2, also known as GLUT2, is a transmembrane facilitated glucose transporter. It plays a crucial role in glucose-stimulated insulin secretion in pancreatic β-cells, glucose-sensitive gene regulation in hepatocytes, and glucose reabsorption in the kidney proximal tubule. It is also involved in glucose-sensing mechanisms in the nervous system, controlling feeding, thermoregulation, and pancreatic islet cell mass and function [1,2].
In mice, biallelic Slc2a2 inactivation causes lethal neonatal diabetes, indicating its essential role in insulin secretion [3]. Whole-body reduced Slc2a2 expression (Slc2a2+/-) in high-fat diet-fed mice leads to an age-related decline in glucose homeostasis, while metformin enhances glucose uptake into the gut from the circulation more significantly in these Slc2a2+/- animals compared to wild-type [4].
In conclusion, Slc2a2 is vital for glucose homeostasis, insulin secretion, and glucose-sensing mechanisms. Mouse models with Slc2a2 inactivation or reduced expression have revealed its significance in neonatal diabetes and age-related glucose regulation, providing valuable insights into the role of Slc2a2 in these disease-related biological processes.
References：
1. Thorens, Bernard. 2014. GLUT2, glucose sensing and glucose homeostasis. In Diabetologia, 58, 221-32. doi:10.1007/s00125-014-3451-1. https://pubmed.ncbi.nlm.nih.gov/25421524/
2. Ghezzi, Chiara, Loo, Donald D F, Wright, Ernest M. 2018. Physiology of renal glucose handling via SGLT1, SGLT2 and GLUT2. In Diabetologia, 61, 2087-2097. doi:10.1007/s00125-018-4656-5. https://pubmed.ncbi.nlm.nih.gov/30132032/
3. Sansbury, F H, Flanagan, S E, Houghton, J A L, Ellard, S, Hattersley, A T. 2012. SLC2A2 mutations can cause neonatal diabetes, suggesting GLUT2 may have a role in human insulin secretion. In Diabetologia, 55, 2381-5. doi:10.1007/s00125-012-2595-0. https://pubmed.ncbi.nlm.nih.gov/22660720/
4. Morrice, Nicola, Vainio, Susanne, Mikkola, Kirsi, Nuutila, Pirjo, Sutherland, Calum. 2023. Metformin increases the uptake of glucose into the gut from the circulation in high-fat diet-fed male mice, which is enhanced by a reduction in whole-body Slc2a2 expression. In Molecular metabolism, 77, 101807. doi:10.1016/j.molmet.2023.101807. https://pubmed.ncbi.nlm.nih.gov/37717665/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen