C57BL/6JCya-Slc31a1em1flox/Cya
Common Name:
Slc31a1-flox
Product ID:
S-CKO-05108
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc31a1-flox
Strain ID
CKOCMP-20529-Slc31a1-B6J-VA
Gene Name
Product ID
S-CKO-05108
Gene Alias
4930445G01Rik; Ctr1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc31a1em1flox/Cya mice (Catalog S-CKO-05108) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000084526
NCBI RefSeq
NM_175090
Target Region
Exon 3
Size of Effective Region
~0.5 kb
Detailed Document
Overview of Gene Research
Slc31a1, also known as CTR1, encodes a protein that functions as a homotrimer for the uptake of dietary copper. It is an important copper transporter in the cell membrane, influencing copper absorption and thus playing a crucial role in maintaining copper homeostasis, which is essential for the activity of numerous copper-containing enzymes involved in various biological processes [4,5,7].
In a study on cardiac fibrosis, fibroblast-specific SLC31A1 deficiency enhanced mitochondrial copper depletion, augmented glycolysis, promoted fibroblast proliferation, and triggered cardiac fibrosis, suggesting that SLC31A1 is crucial in preventing mitochondrial copper depletion-related cardiac fibrosis [3].
In diabetic conditions, excessive advanced glycosylation end products (AGEs) and copper up-regulated the ATF3/SPI1/SLC31A1 signaling, disturbing copper homeostasis and promoting cuproptosis in the myocardium, indicating its role in diabetic myocardial injury [1].
High SLC31A1 expression in breast cancer was related to deregulated immune response, metabolic pathways, and predicted poor prognosis, suggesting its significance in breast cancer prognosis and immune-related processes [2].
In pan-cancer analysis, SLC31A1 expression was significantly different between tumors and normal tissues in nine cancer types, and was linked to immune cell infiltration, immune checkpoint genes, and immunotherapy markers, highlighting its potential in cancer immunotherapy [4].
In diabetic retinopathy, SLC31A1 was up-regulated, and it may be involved in the regulation of cuproptosis through the STAT1-SLC31A1 axis [6].
In cisplatin-induced acute kidney injury, SLC31A1 was up-regulated by ELF3, and its activation triggered cuproptosis through mitochondrial dysfunction, making it a potential therapeutic target to mitigate this injury [8].
In conclusion, Slc31a1 is essential for copper homeostasis. Its dysregulation is associated with various diseases such as cardiac fibrosis, diabetic complications, and cancers. Studies using gene-knockout or conditional-knockout mouse models, as well as other in-vivo studies, have revealed its critical roles in these disease-related biological processes, providing potential therapeutic targets for these conditions.
References：
1. Huo, Shengqi, Wang, Qian, Shi, Wei, Lv, Jiagao, Lin, Li. 2023. ATF3/SPI1/SLC31A1 Signaling Promotes Cuproptosis Induced by Advanced Glycosylation End Products in Diabetic Myocardial Injury. In International journal of molecular sciences, 24, . doi:10.3390/ijms24021667. https://pubmed.ncbi.nlm.nih.gov/36675183/
2. Li, Linrong, Li, Lin, Sun, Qiang. 2022. High expression of cuproptosis-related SLC31A1 gene in relation to unfavorable outcome and deregulated immune cell infiltration in breast cancer: an analysis based on public databases. In BMC bioinformatics, 23, 350. doi:10.1186/s12859-022-04894-6. https://pubmed.ncbi.nlm.nih.gov/35996075/
3. Tu, Bin, Song, Kai, Zhou, Ze-Yu, Zhao, Jian-Yuan, Tao, Hui. 2025. SLC31A1 loss depletes mitochondrial copper and promotes cardiac fibrosis. In European heart journal, , . doi:10.1093/eurheartj/ehaf130. https://pubmed.ncbi.nlm.nih.gov/40048660/
4. Zhang, Pei, Yang, Heqi, Zhu, Kaiguo, Ye, Tinghong, Cao, Dan. 2023. SLC31A1 Identifying a Novel Biomarker with Potential Prognostic and Immunotherapeutic Potential in Pan-Cancer. In Biomedicines, 11, . doi:10.3390/biomedicines11112884. https://pubmed.ncbi.nlm.nih.gov/38001885/
5. Qi, Yue, Yao, Qingqing, Li, Xuanyan, Zhang, Wenwen, Qu, Pengpeng. 2023. Cuproptosis-related gene SLC31A1: prognosis values and potential biological functions in cancer. In Scientific reports, 13, 17790. doi:10.1038/s41598-023-44681-8. https://pubmed.ncbi.nlm.nih.gov/37853210/
6. Hu, Qiang, Zhang, Xue, Huang, Jiayang, Jiang, Bo, Sun, Dawei. 2024. The STAT1-SLC31A1 axis: Potential regulation of cuproptosis in diabetic retinopathy. In Gene, 930, 148861. doi:10.1016/j.gene.2024.148861. https://pubmed.ncbi.nlm.nih.gov/39153705/
7. Xue, Qian, Kang, Rui, Klionsky, Daniel J, Liu, Jinbao, Chen, Xin. 2023. Copper metabolism in cell death and autophagy. In Autophagy, 19, 2175-2195. doi:10.1080/15548627.2023.2200554. https://pubmed.ncbi.nlm.nih.gov/37055935/
8. Qiu, Zhimin, Liu, Qicen, Wang, Ling, Yan, Xiluan, Deng, Huangying. 2024. The copper transporter, SLC31A1, transcriptionally activated by ELF3, imbalances copper homeostasis to exacerbate cisplatin-induced acute kidney injury through mitochondrial dysfunction. In Chemico-biological interactions, 393, 110943. doi:10.1016/j.cbi.2024.110943. https://pubmed.ncbi.nlm.nih.gov/38462020/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen