C57BL/6JCya-Slc7a5em1flox/Cya
Common Name:
Slc7a5-flox
Product ID:
S-CKO-05118
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Slc7a5-flox
Strain ID
CKOCMP-20539-Slc7a5-B6J-VA
Gene Name
Product ID
S-CKO-05118
Gene Alias
4F2LC; D0H16S474E; Gm42049; LAT1; TA1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc7a5em1flox/Cya mice (Catalog S-CKO-05118) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045557
NCBI RefSeq
NM_011404
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Slc7a5, also known as LAT1, is an amino acid transporter. It forms a heterodimeric complex with 4F2hc (SLC3A2), which is crucial for the efficient uptake of essential amino acids and hormones. This transporter promotes cellular growth by stimulating mTORC1 signaling and repressing the integrated stress response (ISR) [5].
Cancer cells rely on increased nutrient uptake, and Slc7a5 is highly expressed in various cancers, related to poor patient prognosis. Pharmacologic inhibition and knockdown/knockout of Slc7a5 suppress cancer cell proliferation and xenograft tumor growth. It inhibits protein synthesis by downregulating mTORC1 and mobilizing the GAAC pathway. In triple-negative breast cancer, knocking down Slc7a5 inhibited cell proliferation, migration, invasion, and increased CD8+ T-cell infiltration. The combination of a Slc7a5 blockade and anti-PD-1 antibody synergistically inhibited tumor progression [1,6]. In gastric cancer, circARID1A promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex, increasing SLC7A5 mRNA stability [3]. In lung cancer, IGF2BP2 enhances SLC7A5 mRNA stability and translation through m6A modification, and this positive feedback loop promotes radioresistance [4].
In conclusion, Slc7a5 is essential for cellular growth through its role in amino acid transport and associated signaling pathways. Studies using knockout or knockdown models have revealed its significant contribution to cancer progression, highlighting its potential as a molecular target for cancer diagnosis and therapeutics. Additionally, it may play a role in enhancing anti-tumor immunity and is involved in the regulation of ferroptosis in head and neck squamous cell carcinoma [1,2,6,7].
References:
1. Kanai, Yoshikatsu. 2021. Amino acid transporter LAT1 (SLC7A5) as a molecular target for cancer diagnosis and therapeutics. In Pharmacology & therapeutics, 230, 107964. doi:10.1016/j.pharmthera.2021.107964. https://pubmed.ncbi.nlm.nih.gov/34390745/
2. Nachef, Marianna, Ali, Alaa Kassim, Almutairi, Saeedah Musaed, Lee, Seung-Hwan. 2021. Targeting SLC1A5 and SLC3A2/SLC7A5 as a Potential Strategy to Strengthen Anti-Tumor Immunity in the Tumor Microenvironment. In Frontiers in immunology, 12, 624324. doi:10.3389/fimmu.2021.624324. https://pubmed.ncbi.nlm.nih.gov/33953707/
3. Ma, Qiang, Yang, Feifei, Huang, Bo, Wang, Shimin, Xiao, Bin. 2022. CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex. In Journal of experimental & clinical cancer research : CR, 41, 251. doi:10.1186/s13046-022-02466-3. https://pubmed.ncbi.nlm.nih.gov/35986300/
4. Zhou, Zhiyuan, Zhang, Bin, Deng, Yue, Wan, Chao, Yang, Kunyu. 2024. FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer. In Journal of experimental & clinical cancer research : CR, 43, 34. doi:10.1186/s13046-024-02959-3. https://pubmed.ncbi.nlm.nih.gov/38281999/
5. Kahlhofer, Jennifer, Teis, David. 2022. The human LAT1-4F2hc (SLC7A5-SLC3A2) transporter complex: Physiological and pathophysiological implications. In Basic & clinical pharmacology & toxicology, 133, 459-472. doi:10.1111/bcpt.13821. https://pubmed.ncbi.nlm.nih.gov/36460306/
6. Huang, Renhong, Wang, Han, Hong, Jin, Shen, Kunwei, Wang, Zheng. 2023. Targeting glutamine metabolic reprogramming of SLC7A5 enhances the efficacy of anti-PD-1 in triple-negative breast cancer. In Frontiers in immunology, 14, 1251643. doi:10.3389/fimmu.2023.1251643. https://pubmed.ncbi.nlm.nih.gov/37731509/
7. Chen, Zilong, Cai, Haoxi, Ye, Weiwei, Cai, Chengfu, Cai, Gengming. 2024. TP63 transcriptionally regulates SLC7A5 to suppress ferroptosis in head and neck squamous cell carcinoma. In Frontiers in immunology, 15, 1445472. doi:10.3389/fimmu.2024.1445472. https://pubmed.ncbi.nlm.nih.gov/39234254/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen