C57BL/6JCya-Sncaem1flox/Cya
Common Name:
Snca-flox
Product ID:
S-CKO-05150
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Snca-flox
Strain ID
CKOCMP-20617-Snca-B6J-VA
Gene Name
Product ID
S-CKO-05150
Gene Alias
NACP; alpha-Syn; alphaSYN
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sncaem1flox/Cya mice (Catalog S-CKO-05150) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000163779
NCBI RefSeq
NM_009221
Target Region
Exon 3~4
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
SNCA, also known as synuclein alpha, encodes α-synuclein, a protein that is the major component of Lewy bodies and Lewy neurites, the pathognomonic hallmarks of Parkinson's disease (PD) [5]. It has been implicated in the etiology of synucleinopathies, and its expression levels seem to be critical for the development of these diseases [4,6].
In PD, abnormal intracellular accumulation of SNCA/α-synuclein occurs. Deficiency of CTSD, a lysosomal protease proposed to be involved in SNCA degradation, has been linked to insoluble SNCA conformers and their transcellular transmission. Recombinant human pro-CTSD enhances SNCA degradation in α-Synucleinopathy models, restoring endo-lysosome and autophagy function [1]. Also, studies show higher levels of SNCA mRNA in sporadic PD post-mortem midbrain tissues and substantia nigra dopaminergic neurons, suggesting that regulating SNCA expression could be an effective treatment approach for PD [2]. Neuronal SNCA transcription during Lewy body formation shows that preserved cellular SNCA expression in non-Lewy body type α-syn cytopathologies might provide a pool for protein aggregation, while successful segregation of disease-associated α-syn is related to SNCA production exhaustion in Lewy bodies, informing LBD therapy development targeting SNCA transcription [3].
In conclusion, SNCA is central to the pathogenesis of synucleinopathies, especially PD. Research on SNCA, including through gene-based models, has enhanced our understanding of its role in these diseases, potentially paving the way for new therapeutic strategies targeting SNCA expression and α-synuclein degradation.
References:
1. Prieto Huarcaya, Susy, Drobny, Alice, Marques, André R A, Saftig, Paul, Zunke, Friederike. 2022. Recombinant pro-CTSD (cathepsin D) enhances SNCA/α-Synuclein degradation in α-Synucleinopathy models. In Autophagy, 18, 1127-1151. doi:10.1080/15548627.2022.2045534. https://pubmed.ncbi.nlm.nih.gov/35287553/
2. Caramiello, Alessio Maria, Pirota, Valentina. 2024. Novel Therapeutic Horizons: SNCA Targeting in Parkinson's Disease. In Biomolecules, 14, . doi:10.3390/biom14080949. https://pubmed.ncbi.nlm.nih.gov/39199337/
3. Kon, Tomoya, Forrest, Shelley L, Lee, Seojin, Lang, Anthony E, Kovacs, Gabor G. 2023. Neuronal SNCA transcription during Lewy body formation. In Acta neuropathologica communications, 11, 185. doi:10.1186/s40478-023-01687-7. https://pubmed.ncbi.nlm.nih.gov/37996943/
4. Chiba-Falek, Ornit. 2017. Structural variants in SNCA gene and the implication to synucleinopathies. In Current opinion in genetics & development, 44, 110-116. doi:10.1016/j.gde.2017.01.014. https://pubmed.ncbi.nlm.nih.gov/28319736/
5. Xu, Wei, Tan, Lan, Yu, Jin-Tai. 2014. Link between the SNCA gene and parkinsonism. In Neurobiology of aging, 36, 1505-18. doi:10.1016/j.neurobiolaging.2014.10.042. https://pubmed.ncbi.nlm.nih.gov/25554495/
6. Tagliafierro, L, Chiba-Falek, O. 2016. Up-regulation of SNCA gene expression: implications to synucleinopathies. In Neurogenetics, 17, 145-57. doi:10.1007/s10048-016-0478-0. https://pubmed.ncbi.nlm.nih.gov/26948950/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen