C57BL/6JCya-Snta1em1flox/Cya
Common Name:
Snta1-flox
Product ID:
S-CKO-05164
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Snta1-flox
Strain ID
CKOCMP-20648-Snta1-B6J-VA
Gene Name
Product ID
S-CKO-05164
Gene Alias
Snt1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snta1em1flox/Cya mice (Catalog S-CKO-05164) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109728
NCBI RefSeq
NM_009228
Target Region
Exon 3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
SNTA1 encodes α-1-syntrophin, a scaffold protein that is part of the dystrophin-associated protein complex. It interacts with SCN5A and nNOS-PMCA4b complex in cardiomyocytes and is involved in regulating ion channels and cardiac excitability. Also, it is related to the perivascular localization of aquaporin-4 (AQP4) in the brain, thus influencing glymphatic solute transport [2,3,4,5].
In human cardiomyocytes, SNTA1-deficiency leads to a hypertrophic phenotype, lower cardiac contractility, weak calcium transient intensity, and lower level of calcium in the sarcoplasmic reticulum, indicating its role in maintaining cardiac calcium homeostasis [1]. In a mouse model lacking perivascular AQP4 localization due to SNTA1 gene deletion, glymphatic CSF tracer influx and interstitial tracer efflux from the brain were slowed, and amyloid β levels increased, suggesting its importance in glymphatic exchange and amyloid β clearance [4].
In conclusion, SNTA1 is crucial for normal cardiac function, especially in maintaining calcium homeostasis, and also plays a role in glymphatic function in the brain. The gene knockout models of SNTA1 have provided valuable insights into its functions and its association with myocardial diseases and Alzheimer's disease-related pathology.
References:
1. Dong, Tao, Zhao, Yan, Jin, Hai-Feng, Chen, Li, Liu, Ji-Cheng. 2022. SNTA1-deficient human cardiomyocytes demonstrate hypertrophic phenotype and calcium handling disorder. In Stem cell research & therapy, 13, 288. doi:10.1186/s13287-022-02955-4. https://pubmed.ncbi.nlm.nih.gov/35773684/
2. Mestre, Humberto, Hablitz, Lauren M, Xavier, Anna Lr, Iliff, Jeffrey J, Nedergaard, Maiken. 2018. Aquaporin-4-dependent glymphatic solute transport in the rodent brain. In eLife, 7, . doi:10.7554/eLife.40070. https://pubmed.ncbi.nlm.nih.gov/30561329/
3. Jimenez-Vazquez, Eric N, Arad, Michael, Macías, Álvaro, Michele, Daniel E, Jalife, José. 2022. SNTA1 gene rescues ion channel function and is antiarrhythmic in cardiomyocytes derived from induced pluripotent stem cells from muscular dystrophy patients. In eLife, 11, . doi:10.7554/eLife.76576. https://pubmed.ncbi.nlm.nih.gov/35762211/
4. Simon, Matthew, Wang, Marie Xun, Ismail, Ozama, Boison, Detlev, Iliff, Jeffrey J. 2022. Loss of perivascular aquaporin-4 localization impairs glymphatic exchange and promotes amyloid β plaque formation in mice. In Alzheimer's research & therapy, 14, 59. doi:10.1186/s13195-022-00999-5. https://pubmed.ncbi.nlm.nih.gov/35473943/
5. Dong, Tao, Zhang, Siyao, Chang, Yun, Jiang, Hong Feng, Lu, Wen-Jing. 2021. The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the Nuclease technology system. In Stem cell research, 51, 102196. doi:10.1016/j.scr.2021.102196. https://pubmed.ncbi.nlm.nih.gov/33524674/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen