C57BL/6JCya-Sox14em1flox/Cya
Common Name:
Sox14-flox
Product ID:
S-CKO-05181
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sox14-flox
Strain ID
CKOCMP-20669-Sox14-B6J-VA
Gene Name
Product ID
S-CKO-05181
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sox14em1flox/Cya mice (Catalog S-CKO-05181) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054819
NCBI RefSeq
NM_011440
Target Region
Exon 1
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Sox14, a member of the SOXB2 subclass of transcription factors, is mainly involved in neural development [2,4,5]. It acts as a transcriptional modulator of cell proliferation and is essential for the initiation of neuronal differentiation in the chick spinal cord [2]. In addition, Sox14 may be associated with the regulation of 20-hydroxyecdysone (20E) signaling during Drosophila metamorphosis [6].
In cervical cancer, Sox14 methylation levels are significantly increased in cancer tissues compared to normal tissues. Hypermethylated Sox14 has the potential to be a biomarker for the screening and early diagnosis of cervical cancer [1]. Moreover, Sox14 promotes the proliferation and invasion of cervical cancer cells through the Wnt/β-catenin pathway, while also activating the p53 signaling pathway to induce apoptosis in cervical carcinoma cell lines [4,5].
In the mouse cerebellum, Sox14 is an exclusive marker of specific inhibitory projection neurons, and ablation of Sox14+ cells leads to impaired motor learning [3]. In Drosophila, Sox14 is necessary and sufficient to mediate dendrite severing during pruning in response to ecdysone signaling [7].
In summary, Sox14 plays crucial roles in neural development, cell proliferation, apoptosis, and disease-related processes. Studies, including those using model organisms like chicks, mice, and Drosophila, have revealed its diverse functions in neuronal differentiation, motor learning, dendrite pruning, and as a potential biomarker and regulator in cervical cancer.
References:
1. Zhao, Jing, Cao, Huiling, Zhang, Wenfan, Shi, Shujuan, Wang, Rong. 2021. SOX14 hypermethylation as a tumour biomarker in cervical cancer. In BMC cancer, 21, 675. doi:10.1186/s12885-021-08406-2. https://pubmed.ncbi.nlm.nih.gov/34098886/
2. Katsuyama, Taiki, Kadoya, Minori, Shirai, Manabu, Sasai, Noriaki. 2021. Sox14 is essential for initiation of neuronal differentiation in the chick spinal cord. In Developmental dynamics : an official publication of the American Association of Anatomists, 251, 350-361. doi:10.1002/dvdy.392. https://pubmed.ncbi.nlm.nih.gov/34181293/
3. Prekop, Hong-Ting, Kroiss, Anna, Rook, Victoria, Delogu, Alessio, Wingate, Richard J T. 2018. Sox14 Is Required for a Specific Subset of Cerebello-Olivary Projections. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 38, 9539-9550. doi:10.1523/JNEUROSCI.1456-18.2018. https://pubmed.ncbi.nlm.nih.gov/30242051/
4. Li, Fei, Wang, Tairen, Tang, Shengjian. 2015. SOX14 promotes proliferation and invasion of cervical cancer cells through Wnt/β-catenin pathway. In International journal of clinical and experimental pathology, 8, 1698-704. doi:. https://pubmed.ncbi.nlm.nih.gov/25973056/
5. Stanisavljevic, Danijela, Petrovic, Isidora, Vukovic, Vladanka, Stevanovic, Milena, Popovic, Jelena. 2017. SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line. In PloS one, 12, e0184686. doi:10.1371/journal.pone.0184686. https://pubmed.ncbi.nlm.nih.gov/28926586/
6. Ritter, Amanda R, Beckstead, Robert B. . Sox14 is required for transcriptional and developmental responses to 20-hydroxyecdysone at the onset of drosophila metamorphosis. In Developmental dynamics : an official publication of the American Association of Anatomists, 239, 2685-94. doi:10.1002/dvdy.22407. https://pubmed.ncbi.nlm.nih.gov/20803583/
7. Kirilly, Daniel, Gu, Ying, Huang, Yafen, Wang, Hongyan, Yu, Fengwei. 2009. A genetic pathway composed of Sox14 and Mical governs severing of dendrites during pruning. In Nature neuroscience, 12, 1497-505. doi:10.1038/nn.2415. https://pubmed.ncbi.nlm.nih.gov/19881505/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen