C57BL/6JCya-Sp1em1flox/Cya
Common Name:
Sp1-flox
Product ID:
S-CKO-05192
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Sp1-flox
Strain ID
CKOCMP-20683-Sp1-B6J-VA
Gene Name
Product ID
S-CKO-05192
Gene Alias
1110003E12Rik; Sp1-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sp1em1flox/Cya mice (Catalog S-CKO-05192) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001326
NCBI RefSeq
NM_013672
Target Region
Exon 1~3
Size of Effective Region
~5.3 kb
Detailed Document
Overview of Gene Research
Sp1, also known as specificity protein 1, is a well-known transcription factor belonging to the Sp family, which also includes Sp2, Sp3 and Sp4 [1]. It activates the transcription of many cellular genes containing CG-rich Sp-binding sites in their promoters and is involved in a variety of essential biological processes such as cell growth, differentiation, apoptosis and carcinogenesis [1]. Sp1 also participates in pathways related to cell proliferation, metastasis, lipid metabolism, inflammation and more, and is of great biological importance [1,2].
In cancer, high levels of Sp1 protein are considered a negative prognostic factor. Compounds that can interfere with Sp1's trans-activating activities may be used in treating tumors that over-express Sp1 [1]. In atherosclerosis, Sp1 is involved in key events like inflammation, lipid metabolism, plaque stability, VSMCs proliferation and endothelial dysfunction [2]. Sp1 also represses distal poly(A) site usage in alternative polyadenylation, and its 3' UTR length regulation in breast cancer may be related to tumorigenic properties [3]. In lung adenocarcinoma, SP1 undergoes phase separation, activates RGS20 expression through super-enhancers, promoting cancer progression, and GSK-J4 can abolish this oncogenic activity [4].
In conclusion, Sp1 is a crucial transcription factor involved in multiple biological processes and various diseases including cancer and atherosclerosis. Understanding its functions through research, although no KO/CKO mouse models are specifically mentioned in the provided references, helps in uncovering the molecular mechanisms of diseases and may provide potential therapeutic targets for these diseases.
References:
1. Vizcaíno, Carolina, Mansilla, Sylvia, Portugal, José. 2015. Sp1 transcription factor: A long-standing target in cancer chemotherapy. In Pharmacology & therapeutics, 152, 111-24. doi:10.1016/j.pharmthera.2015.05.008. https://pubmed.ncbi.nlm.nih.gov/25960131/
2. Jiang, Jie-Feng, Zhou, Zheng-Yang, Liu, Yi-Zhang, Huang, Liang, Zhang, Chi. 2022. Role of Sp1 in atherosclerosis. In Molecular biology reports, 49, 9893-9902. doi:10.1007/s11033-022-07516-9. https://pubmed.ncbi.nlm.nih.gov/35715606/
3. Song, Jingwen, Nabeel-Shah, Syed, Pu, Shuye, Blencowe, Benjamin J, Greenblatt, Jack F. 2022. Regulation of alternative polyadenylation by the C2H2-zinc-finger protein Sp1. In Molecular cell, 82, 3135-3150.e9. doi:10.1016/j.molcel.2022.06.031. https://pubmed.ncbi.nlm.nih.gov/35914531/
4. Shan, Liying, Wang, Wenmeng, Du, Lijuan, Sui, Guangchao, Liu, Fang. 2024. SP1 undergoes phase separation and activates RGS20 expression through super-enhancers to promote lung adenocarcinoma progression. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2401834121. doi:10.1073/pnas.2401834121. https://pubmed.ncbi.nlm.nih.gov/38976739/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen