C57BL/6JCya-Spin1em1flox/Cya
Common Name:
Spin1-flox
Product ID:
S-CKO-05220
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Spin1-flox
Strain ID
CKOCMP-20729-Spin1-B6J-VA
Gene Name
Product ID
S-CKO-05220
Gene Alias
SSEC P; Spin
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Spin1em1flox/Cya mice (Catalog S-CKO-05220) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000095797
NCBI RefSeq
NM_146043
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Spin1, also known as Spindlin1, is a histone methylation reader. It epigenetically controls multiple tumorigenesis-associated signaling pathways like the Wnt, PI3K/AKT, and RET pathways, and is thus of great importance in cancer-related biological processes [1]. It is a transcriptional co-activator with functions in embryonic development and emerging roles in cancer [2].
Functionally, Spin1 depletion inhibits cell proliferation, migration, invasion, and decreases the clonogenic capacity, impairs double-strand break repair, and increases radiosensitivity in non-small cell lung cancer (NSCLC), revealing its role in NSCLC progression and radioresistance [3]. In liver cancer, Spin1 modulates abnormal lipid metabolism by up-regulating FASN and co-activating SREBP1c, enhancing tumor growth [4]. In human seminoma, Spin1 overexpression reduces apoptosis and up-regulates CYCD1, indicating its pro-oncogenic role [5]. In gastric cancer, Spin1 promotes cell proliferation, migration, invasion, and cell cycle progression via the MDM2-p21-E2F1 signaling pathway, and forms a positive feedback loop with E2F1 [6]. Also, Spin1 ablation activates p53, suppresses cell growth, reduces clonogenic ability, and induces apoptosis of human cancer cells by blocking the uL18-MDM2-p53 pathway [7].
In conclusion, Spin1 is a crucial factor in multiple biological and disease-related processes, especially in cancer. Its functions in promoting tumorigenesis, chemoresistance, and radioresistance are revealed through various loss-of-function experiments, highlighting its potential as a therapeutic target for cancers such as NSCLC, liver cancer, seminoma, and gastric cancer.
References:
1. Li, Di, Guo, Jihua, Jia, Rong. 2021. Histone code reader SPIN1 is a promising target of cancer therapy. In Biochimie, 191, 78-86. doi:10.1016/j.biochi.2021.09.002. https://pubmed.ncbi.nlm.nih.gov/34492335/
2. Wang, Yukun, Li, Mengyao, Chen, Yuhan, Liu, Xiuhua, Wu, Chen. 2024. SPIN1 facilitates chemoresistance and HR repair by promoting Tip60 binding to H3K9me3. In EMBO reports, 25, 3970-3989. doi:10.1038/s44319-024-00219-1. https://pubmed.ncbi.nlm.nih.gov/39090319/
3. Zhong, Min, Fang, Zhi, Zou, Juntao, Xiang, Xiaojun, Fang, Ziling. 2024. SPIN1 accelerates tumorigenesis and confers radioresistance in non-small cell lung cancer by orchestrating the FOXO3a/FOXM1 axis. In Cell death & disease, 15, 832. doi:10.1038/s41419-024-07225-0. https://pubmed.ncbi.nlm.nih.gov/39548064/
4. Zhao, Man, Bu, Yanan, Feng, Jinyan, Wang, Jiapei, Zhang, Xiaodong. 2019. SPIN1 triggers abnormal lipid metabolism and enhances tumor growth in liver cancer. In Cancer letters, 470, 54-63. doi:10.1016/j.canlet.2019.11.032. https://pubmed.ncbi.nlm.nih.gov/31790762/
5. Janecki, Damian Mikolaj, Sajek, Marcin, Smialek, Maciej Jerzy, Kurpisz, Maciej, Jaruzelska, Jadwiga. 2018. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. In Oncotarget, 9, 32466-32477. doi:10.18632/oncotarget.25977. https://pubmed.ncbi.nlm.nih.gov/30197756/
6. Lv, Bei-Bei, Ma, Ran-Ran, Chen, Xu, Liu, Hai-Ting, Gao, Peng. 2020. E2F1-activated SPIN1 promotes tumor growth via a MDM2-p21-E2F1 feedback loop in gastric cancer. In Molecular oncology, 14, 2629-2645. doi:10.1002/1878-0261.12778. https://pubmed.ncbi.nlm.nih.gov/32767629/
7. Fang, Ziling, Cao, Bo, Liao, Jun-Ming, Xiong, Jianping, Lu, Hua. 2018. SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer. In eLife, 7, . doi:10.7554/eLife.31275. https://pubmed.ncbi.nlm.nih.gov/29547122/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen