C57BL/6JCya-Sqleem1flox/Cya
Common Name:
Sqle-flox
Product ID:
S-CKO-05265
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sqle-flox
Strain ID
CKOCMP-20775-Sqle-B6J-VA
Gene Name
Product ID
S-CKO-05265
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sqleem1flox/Cya mice (Catalog S-CKO-05265) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022977
NCBI RefSeq
NM_009270
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Sqle, squalene epoxidase, is a key rate-limiting enzyme in cholesterol biosynthesis [2,3,4,5]. It is involved in multiple biological processes. By catalyzing the conversion of squalene to squalene-2,3-epoxide, it is crucial for de novo cholesterol biosynthesis. Cholesterol metabolism, in which Sqle plays a part, is associated with various cellular functions and signaling pathways [1,3,6].
In cancer research, Sqle has been shown to play oncogenic roles. In pancreatic cancer, its inhibition led to squalene accumulation-induced endoplasmic reticulum (ER) stress and apoptosis, while its overexpression promoted cell proliferation, cell cycle progression, and tumor growth in vivo by attenuating ER stress and activating lipid rafts-regulated Src/PI3K/Akt signaling pathway [1]. In bladder cancer, Sqle knockdown inhibited tumor cell proliferation and metastasis through the PTEN/AKT/GSK3β signaling pathway, and P53 was identified as a key molecule in this regulation [2]. In osteosarcoma, silence of Sqle suppressed cell proliferation, migration, and invasion, while overexpression had the opposite effects by activating the TGFβ/SMAD signaling pathway [4].
In conclusion, Sqle is essential for cholesterol metabolism. Through gene-knockout or related functional studies in various cancer models, it has been revealed that Sqle plays significant roles in promoting cancer cell growth, proliferation, and metastasis in pancreatic, bladder, and osteosarcoma cancers. Understanding the function of Sqle provides potential therapeutic targets for these diseases.
References:
1. Xu, Ruiyuan, Song, Jianlu, Ruze, Rexiati, Wang, Chengcheng, Zhao, Yupei. 2023. SQLE promotes pancreatic cancer growth by attenuating ER stress and activating lipid rafts-regulated Src/PI3K/Akt signaling pathway. In Cell death & disease, 14, 497. doi:10.1038/s41419-023-05987-7. https://pubmed.ncbi.nlm.nih.gov/37542052/
2. Zou, Fan, Chen, Wu, Song, Tianbao, Yu, Weimin, Cheng, Fan. 2023. SQLE Knockdown inhibits bladder cancer progression by regulating the PTEN/AKT/GSK3β signaling pathway through P53. In Cancer cell international, 23, 221. doi:10.1186/s12935-023-02997-5. https://pubmed.ncbi.nlm.nih.gov/37770925/
3. Zhang, Jiapeng, Xu, Hang, He, Yirui, Tan, Ping, Wei, Qiang. 2024. Inhibition of KDM4A restricts SQLE transcription and induces oxidative stress imbalance to suppress bladder cancer. In Redox biology, 77, 103407. doi:10.1016/j.redox.2024.103407. https://pubmed.ncbi.nlm.nih.gov/39461328/
4. Song, Qi, He, Lina, Feng, Jing. 2024. SQLE promotes osteosarcoma progression via activating TGFβ/SMAD signaling pathway. In Molecular and cellular probes, 78, 101993. doi:10.1016/j.mcp.2024.101993. https://pubmed.ncbi.nlm.nih.gov/39608425/
5. Xu, Huanji, Zhou, Sheng, Tang, Qiulin, Xia, Hongwei, Bi, Feng. 2020. Cholesterol metabolism: New functions and therapeutic approaches in cancer. In Biochimica et biophysica acta. Reviews on cancer, 1874, 188394. doi:10.1016/j.bbcan.2020.188394. https://pubmed.ncbi.nlm.nih.gov/32698040/
6. You, Weiqiang, Ke, Jia, Chen, Yufeng, Hu, Peishan, Wu, Xiaojian. 2022. SQLE, A Key Enzyme in Cholesterol Metabolism, Correlates With Tumor Immune Infiltration and Immunotherapy Outcome of Pancreatic Adenocarcinoma. In Frontiers in immunology, 13, 864244. doi:10.3389/fimmu.2022.864244. https://pubmed.ncbi.nlm.nih.gov/35720314/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen