C57BL/6JCya-Srebf2em1flox/Cya
Common Name:
Srebf2-flox
Product ID:
S-CKO-05278
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Srebf2-flox
Strain ID
CKOCMP-20788-Srebf2-B6J-VA
Gene Name
Product ID
S-CKO-05278
Gene Alias
SREBP-2; SREBP2; SREBP2gc; bHLHd2; lop13; nuc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Srebf2em1flox/Cya mice (Catalog S-CKO-05278) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023100
NCBI RefSeq
NM_033218
Target Region
Exon 2~3
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Srebf2, short for sterol regulatory element-binding transcription factor 2, is a key regulator of cholesterol metabolism. It belongs to the SREBP family of transcription factors and controls the expression of enzymes involved in cholesterol synthesis [3]. Srebf2-related pathways are crucial for maintaining lipid homeostasis, and genetic models such as gene knockout studies can be used to explore its functions.
In various disease-related research, knockdown of Srebf2 in sorafenib-resistant HepG2 and Huh7 cells resensitized them to sorafenib, suggesting that Srebf2 promotes sorafenib resistance in hepatocellular carcinoma through the SREBF2-STARD4 axis [2]. In endothelial cells, LPS-induced Srebf2 expression, via the TLR4/JNK/c-Jun pathway, led to ER stress and Bax-mediated injury, and miR590-3p could alleviate this by negatively regulating Srebf2 [1]. In polycystic ovary syndrome, the rhythm gene PER1 promoted granulosa cell ferroptosis and abnormal lipid metabolism by inhibiting Srebf2 [4]. Also, in zebrafish, chemical inhibition or gene knockdown of srebf2 decreased optic nerve axon regeneration, indicating its importance in this process [5]. Pharmacological inhibition or RNA interference of Srebf2 attenuated liver cancer initiation in mice with gut flora disequilibrium [6].
In conclusion, Srebf2 is essential for cholesterol metabolism and lipid homeostasis. Model-based research, especially loss-of-function experiments, has revealed its significant roles in diseases such as hepatocellular carcinoma, endothelial cell injury, polycystic ovary syndrome, optic nerve regeneration, and liver cancer initiation. Understanding Srebf2 functions can provide potential therapeutic targets for these diseases.
References:
1. Dong, Gang, Huang, Xiaoquan, Wu, Ling, Tan, Qintian, Chen, Shiyao. 2021. SREBF2 triggers endoplasmic reticulum stress and Bax dysregulation to promote lipopolysaccharide-induced endothelial cell injury. In Cell biology and toxicology, 38, 185-201. doi:10.1007/s10565-021-09593-1. https://pubmed.ncbi.nlm.nih.gov/33677747/
2. Yue, Xuetian, Kong, Youzi, Zhang, Yankun, Liang, Xiaohong, Ma, Chunhong. 2022. SREBF2-STARD4 axis confers sorafenib resistance in hepatocellular carcinoma by regulating mitochondrial cholesterol homeostasis. In Cancer science, 114, 477-489. doi:10.1111/cas.15449. https://pubmed.ncbi.nlm.nih.gov/35642354/
3. Eberlé, Delphine, Hegarty, Bronwyn, Bossard, Pascale, Ferré, Pascal, Foufelle, Fabienne. . SREBP transcription factors: master regulators of lipid homeostasis. In Biochimie, 86, 839-48. doi:. https://pubmed.ncbi.nlm.nih.gov/15589694/
4. Chen, Yuanyuan, Liu, Zhaohua, Chen, Hongmei, Fan, Lang, Luo, Man. 2024. Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167182. doi:10.1016/j.bbadis.2024.167182. https://pubmed.ncbi.nlm.nih.gov/38653359/
5. Hu, Mengming, Veldman, Matthew B. 2025. Srebf2 mediates successful optic nerve axon regeneration via the mevalonate synthesis pathway. In Molecular neurodegeneration, 20, 28. doi:10.1186/s13024-025-00807-2. https://pubmed.ncbi.nlm.nih.gov/40045384/
6. Chen, Wen, Wen, Liang, Bao, Yingying, Li, Muchun, Liang, Tingbo. 2022. Gut flora disequilibrium promotes the initiation of liver cancer by modulating tryptophan metabolism and up-regulating SREBP2. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2203894119. doi:10.1073/pnas.2203894119. https://pubmed.ncbi.nlm.nih.gov/36534812/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen