C57BL/6JCya-Vcpkmtem1flox/Cya
Common Name:
Vcpkmt-flox
Product ID:
S-CKO-05284
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Vcpkmt-flox
Strain ID
CKOCMP-207965-Vcpkmt-B6J-VA
Gene Name
Product ID
S-CKO-05284
Gene Alias
Gm71; Mettl21d; Mettl221d; VCP-KMT
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vcpkmtem1flox/Cya mice (Catalog S-CKO-05284) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000058639
NCBI RefSeq
NM_001033236
Target Region
Exon 4~6
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Vcpkmt, also known as METTL21D, is a valosin-containing protein lysine methyltransferase. It catalyzes the trimethylation of Lys315 of p97 (also known as valosin-containing protein [VCP]), an ATPase involved in diverse cellular processes like membrane fusion and proteolysis [1,3,4,5].
A constitutive knockout mouse targeting exon 1-4 of the Vcpkmt gene was generated. In Vcpkmt-/-mice, the methylation of lysine 315 in VCP was completely abolished. Vcpkmt-/-mice were viable, fertile, and had no obvious pathological phenotype, suggesting that VCPKMT is not essential for development or survival under unstressed conditions [2].
In summary, Vcpkmt is an enzyme required for the methylation of K315 of VCP in vivo. The Vcpkmt KO mouse model has shown that this methylation is dispensable for the basic development and survival of mice, providing insights into the role of Vcpkmt-mediated VCP methylation in normal physiological processes [2].
References：
1. Nguyen, Thang Quyet, Koh, Seri, Kwon, Jiin, Kang, Wonchull, Yang, Jin Kuk. 2023. Structural basis for recognition and methylation of p97 by METTL21D, a valosin-containing protein lysine methyltransferase. In iScience, 26, 107222. doi:10.1016/j.isci.2023.107222. https://pubmed.ncbi.nlm.nih.gov/37456834/
2. Fusser, Markus, Kernstock, Stefan, Aileni, Vinay Kumar, Falnes, Pål Ø, Klungland, Arne. 2015. Lysine Methylation of the Valosin-Containing Protein (VCP) Is Dispensable for Development and Survival of Mice. In PloS one, 10, e0141472. doi:10.1371/journal.pone.0141472. https://pubmed.ncbi.nlm.nih.gov/26544960/
3. Petrović, Saša, Roske, Yvette, Rami, Biria, Panáková, Daniela, Heinemann, Udo. 2023. Structural remodeling of AAA+ ATPase p97 by adaptor protein ASPL facilitates posttranslational methylation by METTL21D. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2208941120. doi:10.1073/pnas.2208941120. https://pubmed.ncbi.nlm.nih.gov/36656859/
4. Aileni, Vinay Kumar, Davydova, Erna, Moen, Anders, Falnes, Pål Ø. 2017. A System for Enzymatic Lysine Methylation in a Desired Sequence Context. In ACS omega, 2, 462-469. doi:10.1021/acsomega.6b00486. https://pubmed.ncbi.nlm.nih.gov/28357416/
5. Kernstock, Stefan, Davydova, Erna, Jakobsson, Magnus, Egge-Jacobsen, Wolfgang, Falnes, Pål Ø. . Lysine methylation of VCP by a member of a novel human protein methyltransferase family. In Nature communications, 3, 1038. doi:10.1038/ncomms2041. https://pubmed.ncbi.nlm.nih.gov/22948820/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen