C57BL/6NCya-Srp14em1flox/Cya
Common Name:
Srp14-flox
Product ID:
S-CKO-05296
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Srp14-flox
Strain ID
CKOCMP-20813-Srp14-B6N-VA
Gene Name
Product ID
S-CKO-05296
Gene Alias
14kDa
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Srp14em1flox/Cya mice (Catalog S-CKO-05296) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000009693
NCBI RefSeq
NM_009273
Target Region
Exon 1~3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Srp14, a crucial protein subunit of the signal recognition particle (SRP), is essential for co-translational translocation to the endoplasmic reticulum. It forms a heterodimer with SRP9, playing a key role in 7SL RNA processing and Alu retrotransposition, and is also involved in the transcriptional regulation of 7SL and BC200 RNA [1,2]. Additionally, it participates in diverse biological processes such as the unfolded protein response, HIV-1 Tat mRNA processing, and is associated with diseases like endometriosis and idiopathic inflammatory myopathies [3,4,7].
In the context of the unfolded protein response, SRP14 reduction, which requires PRKR-like ER kinase (PERK)-mediated eukaryotic translation initiation factor 2α (eIF2α) phosphorylation, leads to reduced translocation of fusion proteins and endogenous cathepsin D, suggesting that the PERK-SRP14 axis-mediated translocational attenuation is a protective measure for the response [3]. In HIV-1 latency, knockdown of SRP14 negatively affects tat mRNA processing, translation, and Tat-mediated transactivation, increasing latent infection, while its overexpression in resting CD4+ T-cells can reverse latency and induce virus production [5]. Regarding porcine reproductive and respiratory syndrome virus (PRRSV) infection, miR-10a downregulates SRP14 expression to inhibit viral replication, and the IRF8-miR-10a-SRP14 regulatory pathway is identified as a new antiviral strategy [6].
In conclusion, Srp14 is vital for multiple biological processes, especially those related to protein translocation and RNA regulation. Its study in various disease-related models, such as in ER stress, HIV-1 latency, and PRRSV infection, provides insights into disease mechanisms, potentially guiding the development of new treatments.
References:
1. Gussakovsky, Daniel, Booy, Evan P, Brown, Mira J F, McKenna, Sean A. 2023. Nuclear SRP9/SRP14 heterodimer transcriptionally regulates 7SL and BC200 RNA expression. In RNA (New York, N.Y.), 29, 1185-1200. doi:10.1261/rna.079649.123. https://pubmed.ncbi.nlm.nih.gov/37156570/
2. Gussakovsky, Daniel, Black, Nicole A, Booy, Evan P, McKenna, Sean A. 2024. The role of SRP9/SRP14 in regulating Alu RNA. In RNA biology, 21, 1-12. doi:10.1080/15476286.2024.2430817. https://pubmed.ncbi.nlm.nih.gov/39563162/
3. Liu, Yaofu, Gu, Yuexi, Chen, Ying, Fang, Shengyun, Yang, Yili. 2024. Translocational attenuation mediated by the PERK-SRP14 axis is a protective mechanism of unfolded protein response. In Cell reports, 43, 114402. doi:10.1016/j.celrep.2024.114402. https://pubmed.ncbi.nlm.nih.gov/38943644/
4. Rahmioglu, Nilufer, Mortlock, Sally, Ghiasi, Marzieh, Morris, Andrew P, Zondervan, Krina T. 2023. The genetic basis of endometriosis and comorbidity with other pain and inflammatory conditions. In Nature genetics, 55, 423-436. doi:10.1038/s41588-023-01323-z. https://pubmed.ncbi.nlm.nih.gov/36914876/
5. Khoury, Georges, Lee, Michelle Y, Ramarathinam, Sri H, Lewin, Sharon R, Purcell, Damian F J. 2021. The RNA-Binding Proteins SRP14 and HMGB3 Control HIV-1 Tat mRNA Processing and Translation During HIV-1 Latency. In Frontiers in genetics, 12, 680725. doi:10.3389/fgene.2021.680725. https://pubmed.ncbi.nlm.nih.gov/34194479/
6. Zheng, Zifang, Fu, Xiali, Ling, Xue, Zheng, Haixue, Xiao, Shuqi. 2022. Host Cells Actively Resist Porcine Reproductive and Respiratory Syndrome Virus Infection via the IRF8-MicroRNA-10a-SRP14 Regulatory Pathway. In Journal of virology, 96, e0000322. doi:10.1128/jvi.00003-22. https://pubmed.ncbi.nlm.nih.gov/35293774/
7. Xiao, Yizhi, Xie, Shasha, Li, Hong-Dong, Li, Yisha, Luo, Hui. 2024. Characterised intron retention profiles in muscle tissue of idiopathic inflammatory myopathy subtypes. In Annals of the rheumatic diseases, 83, 901-914. doi:10.1136/ard-2023-225035. https://pubmed.ncbi.nlm.nih.gov/38302260/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen