C57BL/6JCya-Dot1lem1flox/Cya
Common Name:
Dot1l-flox
Product ID:
S-CKO-05319
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dot1l-flox
Strain ID
CKOCMP-208266-Dot1l-B6J-VA
Gene Name
Product ID
S-CKO-05319
Gene Alias
A630076O07; Dot1; KMT4; mDot1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dot1lem1flox/Cya mice (Catalog S-CKO-05319) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105336
NCBI RefSeq
NM_199322
Target Region
Exon 5
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Dot1l, short for disruptor of telomeric silencing 1-like protein, is a lysine methyltransferase. It is the sole enzyme responsible for histone three lysine 79 (H3K79) mono -, di -, and tri-methylation. This methylation is involved in multiple cellular processes such as cell cycle progression, DNA damage response, and development. It plays a significant role in normal hematopoiesis, and its aberrant activity is associated with hematopoietic malignancies, especially those with high HOX gene expression [4].
In triple-negative breast cancer (TNBC), inhibition of Dot1l by EPZ-5676 reduces tumor-initiating stem cells and metastasis in xenografts generated from ALDH1+ TNBC cells. Dot1l maintains MYC expression and self-renewal in ALDH1+ cells and governs pathways like oxidative phosphorylation, cMyc targets, DNA damage response, and WNT activation in these cells [1].
In ovarian cancer, the PARP1-Dot1l transcription axis drives acquired resistance to PARP inhibitor. Upregulated Dot1l expression in PARPi-resistant tissues is associated with diminished survival. Combining a Dot1l inhibitor with PARPi shows a synergistic effect [2].
In atherosclerosis, Dot1l inactivation in vascular smooth muscle cells (VSMCs) via an inducible, tissue-specific knockout mouse model significantly reduces disease progression. Dot1l and its induced H3K79me2 mark directly regulate the transcription of Nf-κB-1 and-2 in VSMCs [3].
In summary, Dot1l is crucial for normal hematopoiesis and is involved in multiple disease processes. Gene knockout models in different tissues have revealed its role in cancer metastasis, drug resistance, and atherosclerosis development. These findings highlight Dot1l as a potential therapeutic target for various diseases.
References:
1. Kurani, Hetakshi, Razavipour, Seyedeh Fatemeh, Harikumar, Kuzhuvelil B, Wahlestedt, Claes, Slingerland, Joyce. . DOT1L Is a Novel Cancer Stem Cell Target for Triple-Negative Breast Cancer. In Clinical cancer research : an official journal of the American Association for Cancer Research, 28, 1948-1965. doi:10.1158/1078-0432.CCR-21-1299. https://pubmed.ncbi.nlm.nih.gov/35135840/
2. Liu, Chaohua, Li, Jiana, Xu, Fei, Wu, Xiaohua, Chen, Xiaojun. 2024. PARP1-DOT1L transcription axis drives acquired resistance to PARP inhibitor in ovarian cancer. In Molecular cancer, 23, 111. doi:10.1186/s12943-024-02025-8. https://pubmed.ncbi.nlm.nih.gov/38778348/
3. Farina, Floriana Maria, Serio, Simone, Hall, Ignacio Fernando, Quintavalle, Manuela, Elia, Leonardo. . The epigenetic enzyme DOT1L orchestrates vascular smooth muscle cell-monocyte crosstalk and protects against atherosclerosis via the NF-κB pathway. In European heart journal, 43, 4562-4576. doi:10.1093/eurheartj/ehac097. https://pubmed.ncbi.nlm.nih.gov/35292818/
4. Arnold, Olivia, Barbosa, Karina, Deshpande, Aniruddha J, Zhu, Nan. 2022. The Role of DOT1L in Normal and Malignant Hematopoiesis. In Frontiers in cell and developmental biology, 10, 917125. doi:10.3389/fcell.2022.917125. https://pubmed.ncbi.nlm.nih.gov/35712672/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen