C57BL/6JCya-Sgms1em1flox/Cya
Common Name:
Sgms1-flox
Product ID:
S-CKO-05334
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sgms1-flox
Strain ID
CKOCMP-208449-Sgms1-B6J-VA
Gene Name
Product ID
S-CKO-05334
Gene Alias
9530058O11Rik; Mob; Sms1; Sor1; Tmem23
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sgms1em1flox/Cya mice (Catalog S-CKO-05334) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000142618
NCBI RefSeq
NM_001168525
Target Region
Exon 6
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Sgms1, encoding sphingomyelin synthase 1, is an enzyme in the sphingosine-1-phosphate signalling pathway. It is involved in synthesizing sphingomyelin and diacylglycerol from phosphatidylcholine and ceramide, determining its participation in regulating intracellular vesicular transport, cholesterol metabolism, cell proliferation, apoptosis, etc [4].
In mice, the Sgms1tm1b allele, with exon 7 knocked out, led to a complete lack of detectable Sgms1 transcript in the inner ear. Sgms1tm1b homozygotes initially had normal auditory brainstem response thresholds but then experienced progressive hearing loss, along with a reduced endocochlear potential [3]. Overexpressing Sgms1 could counteract the abnormality caused by Mettl3 deficiency in the liver, which was characterized by toxic ceramide accumulation, mitochondrial damage, and elevated endoplasmic reticulum stress [2]. Also, SGMS1 addition accelerated mesenchymal stem cell (MSC) osteogenic differentiation, while its silencing suppressed this process. SGMS1 overexpression inhibited ceramide and promoted sphingomyelin levels, enhancing phosphorylated Akt, Runx2, and VEGF levels, and promoting osteogenic-angiogenic coupling [1].
In conclusion, Sgms1 is crucial for multiple biological processes. Its knockout mouse models have revealed its role in hearing function, post-natal liver development, and osteogenic-angiogenic coupling. Understanding Sgms1 through these models provides insights into related disease mechanisms such as hearing loss, liver development disorders, and skeletal dysplasia, potentially guiding the development of therapeutic strategies.
References:
1. Yang, Kai, Luan, Ying-Yi, Wang, Shan, Zhang, Dong-Liang, Yin, Cheng-Hong. 2024. SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway. In iScience, 27, 109358. doi:10.1016/j.isci.2024.109358. https://pubmed.ncbi.nlm.nih.gov/38544565/
2. Wang, Shiguan, Chen, Shanze, Sun, Jianfeng, Heikenwalder, Mathias, Yuan, Detian. 2023. m6A modification-tuned sphingolipid metabolism regulates postnatal liver development in male mice. In Nature metabolism, 5, 842-860. doi:10.1038/s42255-023-00808-9. https://pubmed.ncbi.nlm.nih.gov/37188818/
3. Chen, Jing, Lewis, Morag A, Wai, Alisa, Ingham, Neil J, Steel, Karen P. 2024. A new mutation of Sgms1 causes gradual hearing loss associated with a reduced endocochlear potential. In Hearing research, 451, 109091. doi:10.1016/j.heares.2024.109091. https://pubmed.ncbi.nlm.nih.gov/39067415/
4. Filippenkov, I B, Dergunova, L V, Rozhkova, A V, Sudarkina, O Yu, Limborska, S A. . PECULIARITIES OF THE STRUCTURE AND EXPRESSION OF HUMAN SPHINGOMYELIN SYNTHASE 1 GENE (SGMS1). In Tsitologiia, 58, 267-71. doi:. https://pubmed.ncbi.nlm.nih.gov/30191692/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen