C57BL/6JCya-Stat3em1flox/Cya
Common Name
Stat3-flox
Product ID
S-CKO-05338
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-20848-Stat3-B6J-VA
Status
When using this mouse strain in a publication, please cite “Stat3-flox Mouse (Catalog S-CKO-05338) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Stat3-flox
Strain ID
CKOCMP-20848-Stat3-B6J-VA
Gene Name
Product ID
S-CKO-05338
Gene Alias
Aprf, 1110034C02Rik
Background
C57BL/6JCya
Gene Full Name
signal transducer and activator of transcription 3
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000127638
NCBI Transcript ID
NM_213659
Target Region
Exon 3~4
Size of Effective Region
~1.4 kb
Overview of Gene Research
Stat3, short for signal transducer and activator of transcription 3, is a nuclear transcription factor. It is a key member of the JAK/STAT signaling pathway. STAT3 regulates genes involved in cell cycle, cell survival, and immune response [4]. It has a crucial role in numerous biological processes and is associated with various diseases [1-3, 5-7].
STAT3 is broadly hyperactivated in the tumor ecosystem, both in cancer and non-cancerous cells. It inhibits the expression of immune activation regulators and promotes immunosuppressive factor production, making targeting its signaling pathway a promising cancer therapeutic strategy [1]. In liver fibrosis, its activation can have anti-or pro-inflammatory roles [2]. In breast cancer, overexpressed and constitutively activated STAT3 is involved in progression, proliferation, metastasis, and chemoresistance [3]. In pancreatic cancer, STAT3 binds to the GPX4 promoter region to promote its transcription, and thiostrepton can block GPX4 expression by regulating STAT3, inducing ferroptosis [5]. In breast cancer, PRMT6-mediated methylation of STAT3 at arginine 729 is important for its membrane localization, phosphorylation, and cancer cell metastasis [6]. In kidney disease, dysregulated STAT3 activation is detrimental, and inhibiting its activity has salutary benefits in some kidney disease models [7].
In conclusion, Stat3 is essential in regulating cell-related functions and immune responses. Through studies including gene knockout or conditional knockout mouse models (implied by genetic knockout and manipulation studies in kidney disease [7]), its role in cancer, liver fibrosis, and kidney disease has been revealed. Understanding Stat3's functions contributes to developing new therapeutic strategies for these diseases.
References：
1. Zou, Sailan, Tong, Qiyu, Liu, Bowen, Tian, Yan, Fu, Xianghui. 2020. Targeting STAT3 in Cancer Immunotherapy. In Molecular cancer, 19, 145. doi:10.1186/s12943-020-01258-7. https://pubmed.ncbi.nlm.nih.gov/32972405/
2. Zhao, Jie, Qi, Yong-Fen, Yu, Yan-Rong. 2020. STAT3: A key regulator in liver fibrosis. In Annals of hepatology, 21, 100224. doi:10.1016/j.aohep.2020.06.010. https://pubmed.ncbi.nlm.nih.gov/32702499/
3. Ma, Jia-Hui, Qin, Li, Li, Xia. 2020. Role of STAT3 signaling pathway in breast cancer. In Cell communication and signaling : CCS, 18, 33. doi:10.1186/s12964-020-0527-z. https://pubmed.ncbi.nlm.nih.gov/32111215/
4. Butturini, Elena, Carcereri de Prati, Alessandra, Mariotto, Sofia. 2020. Redox Regulation of STAT1 and STAT3 Signaling. In International journal of molecular sciences, 21, . doi:10.3390/ijms21197034. https://pubmed.ncbi.nlm.nih.gov/32987855/
5. Zhang, Weifan, Gong, Mengyuan, Zhang, Wunai, Ma, Qingyong, Wang, Zheng. 2022. Thiostrepton induces ferroptosis in pancreatic cancer cells through STAT3/GPX4 signalling. In Cell death & disease, 13, 630. doi:10.1038/s41419-022-05082-3. https://pubmed.ncbi.nlm.nih.gov/35859150/
6. Chen, Qianzhi, Hu, Qingyi, Chen, Yan, Li, Lei, Li, Junjun. 2023. PRMT6 methylation of STAT3 regulates tumor metastasis in breast cancer. In Cell death & disease, 14, 655. doi:10.1038/s41419-023-06148-6. https://pubmed.ncbi.nlm.nih.gov/37813837/
7. Pace, Jesse, Paladugu, Praharshasai, Das, Bhaskar, He, John C, Mallipattu, Sandeep K. 2019. Targeting STAT3 signaling in kidney disease. In American journal of physiology. Renal physiology, 316, F1151-F1161. doi:10.1152/ajprenal.00034.2019. https://pubmed.ncbi.nlm.nih.gov/30943069/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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