C57BL/6JCya-Stat3em1flox/Cya
Common Name:
Stat3-flox
Product ID:
S-CKO-05338
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Stat3-flox
Strain ID
CKOCMP-20848-Stat3-B6J-VA
Gene Name
Product ID
S-CKO-05338
Gene Alias
1110034C02Rik; Aprf
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stat3em1flox/Cya mice (Catalog S-CKO-05338) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000127638
NCBI RefSeq
NM_213659
Target Region
Exon 3~4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Stat3, short for signal transducer and activator of transcription 3, is a nuclear transcription factor. It is a key member of the JAK/STAT signaling pathway. STAT3 regulates genes involved in cell cycle, cell survival, and immune response [4]. It has a crucial role in numerous biological processes and is associated with various diseases [1-3, 5-7].
STAT3 is broadly hyperactivated in the tumor ecosystem, both in cancer and non-cancerous cells. It inhibits the expression of immune activation regulators and promotes immunosuppressive factor production, making targeting its signaling pathway a promising cancer therapeutic strategy [1]. In liver fibrosis, its activation can have anti-or pro-inflammatory roles [2]. In breast cancer, overexpressed and constitutively activated STAT3 is involved in progression, proliferation, metastasis, and chemoresistance [3]. In pancreatic cancer, STAT3 binds to the GPX4 promoter region to promote its transcription, and thiostrepton can block GPX4 expression by regulating STAT3, inducing ferroptosis [5]. In breast cancer, PRMT6-mediated methylation of STAT3 at arginine 729 is important for its membrane localization, phosphorylation, and cancer cell metastasis [6]. In kidney disease, dysregulated STAT3 activation is detrimental, and inhibiting its activity has salutary benefits in some kidney disease models [7].
In conclusion, Stat3 is essential in regulating cell-related functions and immune responses. Through studies including gene knockout or conditional knockout mouse models (implied by genetic knockout and manipulation studies in kidney disease [7]), its role in cancer, liver fibrosis, and kidney disease has been revealed. Understanding Stat3's functions contributes to developing new therapeutic strategies for these diseases.
References:
1. Zou, Sailan, Tong, Qiyu, Liu, Bowen, Tian, Yan, Fu, Xianghui. 2020. Targeting STAT3 in Cancer Immunotherapy. In Molecular cancer, 19, 145. doi:10.1186/s12943-020-01258-7. https://pubmed.ncbi.nlm.nih.gov/32972405/
2. Zhao, Jie, Qi, Yong-Fen, Yu, Yan-Rong. 2020. STAT3: A key regulator in liver fibrosis. In Annals of hepatology, 21, 100224. doi:10.1016/j.aohep.2020.06.010. https://pubmed.ncbi.nlm.nih.gov/32702499/
3. Ma, Jia-Hui, Qin, Li, Li, Xia. 2020. Role of STAT3 signaling pathway in breast cancer. In Cell communication and signaling : CCS, 18, 33. doi:10.1186/s12964-020-0527-z. https://pubmed.ncbi.nlm.nih.gov/32111215/
4. Butturini, Elena, Carcereri de Prati, Alessandra, Mariotto, Sofia. 2020. Redox Regulation of STAT1 and STAT3 Signaling. In International journal of molecular sciences, 21, . doi:10.3390/ijms21197034. https://pubmed.ncbi.nlm.nih.gov/32987855/
5. Zhang, Weifan, Gong, Mengyuan, Zhang, Wunai, Ma, Qingyong, Wang, Zheng. 2022. Thiostrepton induces ferroptosis in pancreatic cancer cells through STAT3/GPX4 signalling. In Cell death & disease, 13, 630. doi:10.1038/s41419-022-05082-3. https://pubmed.ncbi.nlm.nih.gov/35859150/
6. Chen, Qianzhi, Hu, Qingyi, Chen, Yan, Li, Lei, Li, Junjun. 2023. PRMT6 methylation of STAT3 regulates tumor metastasis in breast cancer. In Cell death & disease, 14, 655. doi:10.1038/s41419-023-06148-6. https://pubmed.ncbi.nlm.nih.gov/37813837/
7. Pace, Jesse, Paladugu, Praharshasai, Das, Bhaskar, He, John C, Mallipattu, Sandeep K. 2019. Targeting STAT3 signaling in kidney disease. In American journal of physiology. Renal physiology, 316, F1151-F1161. doi:10.1152/ajprenal.00034.2019. https://pubmed.ncbi.nlm.nih.gov/30943069/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen