C57BL/6NCya-Stat5bem1flox/Cya
Common Name:
Stat5b-flox
Product ID:
S-CKO-05341
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Stat5b-flox
Strain ID
CKOCMP-20851-Stat5b-B6N-VA
Gene Name
Product ID
S-CKO-05341
Gene Alias
--
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Stat5bem1flox/Cya mice (Catalog S-CKO-05341) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107358
NCBI RefSeq
NM_011489
Target Region
Exon 5
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Stat5b, a member of the Signal Transducer and Activator of Transcription (STAT)-5 proteins, is crucial in immune regulation, homeostasis, and the development and function of several hematopoietic cells. It is involved in pathways related to cell development, proliferation, and survival, such as the GH-GHR-STAT5b cascade regulating IGF-I production [1,3,5].
In murine cardiomyocytes, genetic disruption of the NRG-1/ERBB4 pathway reduces STAT5b activation and transcription of its target genes, and loss of Stat5b ablates NRG-1-induced cardiomyocyte hypertrophy [2]. In zebrafish embryos, Nuclease technology-mediated knockdown of stat5b results in reduced myocardial growth and cardiac function [2]. In human B-cells, CRISPR-mediated STAT5B deletion from B-cell lines and study of primary lymphocytes from individuals with germline STAT5B mutations revealed that STAT5B restrains IL-21-mediated B-cell differentiation to maintain humoral immune homeostasis [4].
In conclusion, Stat5b is essential in multiple biological processes including immune regulation, myocardial growth, and B-cell differentiation. Gene-knockout models in mice and zebrafish, as well as studies on human cells with STAT5B mutations, have provided insights into its role in diseases such as growth failure, immunodeficiency, and potentially heart-related conditions [1,2,3,4,5].
References：
1. Smith, Madison R, Satter, Lisa R Forbes, Vargas-Hernández, Alexander. 2023. STAT5b: A master regulator of key biological pathways. In Frontiers in immunology, 13, 1025373. doi:10.3389/fimmu.2022.1025373. https://pubmed.ncbi.nlm.nih.gov/36755813/
2. Vaparanta, Katri, Jokilammi, Anne, Paatero, Ilkka, Taimen, Pekka, Elenius, Klaus. 2023. STAT5b is a key effector of NRG-1/ERBB4-mediated myocardial growth. In EMBO reports, 24, e56689. doi:10.15252/embr.202256689. https://pubmed.ncbi.nlm.nih.gov/37009825/
3. Hwa, Vivian. 2015. STAT5B deficiency: Impacts on human growth and immunity. In Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society, 28, 16-20. doi:10.1016/j.ghir.2015.12.006. https://pubmed.ncbi.nlm.nih.gov/26703237/
4. Pelham, Simon J, Caldirola, Maria Soledad, Avery, Danielle T, Ma, Cindy S, Tangye, Stuart G. 2022. STAT5B restrains human B-cell differentiation to maintain humoral immune homeostasis. In The Journal of allergy and clinical immunology, 150, 931-946. doi:10.1016/j.jaci.2022.04.011. https://pubmed.ncbi.nlm.nih.gov/35469842/
5. Hwa, Vivian, Nadeau, Kari, Wit, Jan M, Rosenfeld, Ron G. . STAT5b deficiency: lessons from STAT5b gene mutations. In Best practice & research. Clinical endocrinology & metabolism, 25, 61-75. doi:10.1016/j.beem.2010.09.003. https://pubmed.ncbi.nlm.nih.gov/21396575/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen