C57BL/6JCya-Nek11em1flox/Cya
Common Name:
Nek11-flox
Product ID:
S-CKO-05347
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nek11-flox
Strain ID
CKOCMP-208583-Nek11-B6J-VA
Gene Name
Product ID
S-CKO-05347
Gene Alias
4932416N14Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nek11em1flox/Cya mice (Catalog S-CKO-05347) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038648
NCBI RefSeq
NM_172461
Target Region
Exon 5
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
NEK11, a member of the never in mitosis gene A (NIMA) family of serine/threonine kinases, is involved in multiple biological processes. It plays a crucial role in the DNA damage response, enforcing the G2/M cell cycle checkpoint. Specifically, it controls the degradation of CDC25A, a key CDK activator, by directly phosphorylating it, which is essential for the cell to prevent division when DNA lesions are present. Additionally, it may be associated with cell cycle-related processes during meiosis and DNA replication [3,4,5].
In a Dutch family with melanoma, a rare, nonsense variant in the NEK11 gene (c.1120C>T, p.Arg374Ter) was identified. This variant cosegregated in all five affected members, and somatic loss of the wild-type allele was demonstrated in a melanoma from a variant carrier. Functional analyses showed that this mutation led to loss-of-function through protein instability, suggesting NEK11 as a melanoma susceptibility gene [1]. In mouse oocytes, knockdown of Nek11 by siRNA microinjection did not affect germinal vesicle breakdown (GVBD) and the first polar body extrusion but caused the formation of 2-cell-like eggs, indicating its role in regulating asymmetric cell division during oocyte meiotic maturation [2]. In HCT116 colorectal cancer cells, RNAi-mediated depletion of Nek11 prevented the G2/M arrest induced by genotoxic agents like ionizing radiation or irinotecan and promoted p53-dependent apoptosis, with Nek11S having an important role in the DNA damage response [6].
In conclusion, NEK11 is essential in processes such as the DNA damage response, cell cycle regulation during meiosis, and may be involved in disease conditions like melanoma and colorectal cancer. Studies using gene-knockdown models in cells and identification of disease-associated variants in families have provided insights into its role in these biological processes and diseases.
References:
1. Christodoulou, Eirini, van Doorn, Remco, Visser, Mijke, Jochemsen, Aart, Gruis, Nelleke. 2019. NEK11 as a candidate high-penetrance melanoma susceptibility gene. In Journal of medical genetics, 57, 203-210. doi:10.1136/jmedgenet-2019-106134. https://pubmed.ncbi.nlm.nih.gov/31704778/
2. Guo, Lei, Wang, Zhen-Bo, Wang, Hong-Hui, Hou, Yi, Sun, Qing-Yuan. 2016. Nek11 regulates asymmetric cell division during mouse oocyte meiotic maturation. In Biochemical and biophysical research communications, 474, 667-672. doi:10.1016/j.bbrc.2016.05.002. https://pubmed.ncbi.nlm.nih.gov/27150633/
3. Sørensen, Claus Storgaard, Melixetian, Marina, Klein, Ditte Kjaersgaard, Helin, Kristian. 2010. NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling. In Cell cycle (Georgetown, Tex.), 9, 450-5. doi:. https://pubmed.ncbi.nlm.nih.gov/20090422/
4. Melixetian, Marina, Klein, Ditte Kjaersgaard, Sørensen, Claus Storgaard, Helin, Kristian. 2009. NEK11 regulates CDC25A degradation and the IR-induced G2/M checkpoint. In Nature cell biology, 11, 1247-53. doi:10.1038/ncb1969. https://pubmed.ncbi.nlm.nih.gov/19734889/
5. Noguchi, Kohji, Fukazawa, Hidesuke, Murakami, Yuko, Uehara, Yoshimasa. 2002. Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses. In The Journal of biological chemistry, 277, 39655-65. doi:. https://pubmed.ncbi.nlm.nih.gov/12154088/
6. Sabir, Sarah R, Sahota, Navdeep K, Jones, George D D, Fry, Andrew M. 2015. Loss of Nek11 Prevents G2/M Arrest and Promotes Cell Death in HCT116 Colorectal Cancer Cells Exposed to Therapeutic DNA Damaging Agents. In PloS one, 10, e0140975. doi:10.1371/journal.pone.0140975. https://pubmed.ncbi.nlm.nih.gov/26501353/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen