C57BL/6JCya-Cblbem1flox/Cya
Common Name:
Cblb-flox
Product ID:
S-CKO-05360
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cblb-flox
Strain ID
CKOCMP-208650-Cblb-B6J-VA
Gene Name
Product ID
S-CKO-05360
Gene Alias
Cbl-b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cblbem1flox/Cya mice (Catalog S-CKO-05360) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114471
NCBI RefSeq
NM_001033238
Target Region
Exon 5
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Cblb, short for Casitas B-lineage lymphoma proto-oncogene B, is an E3 ubiquitin ligase. It plays a key role in downregulating positive signaling cascades by ubiquitinating proteins downstream of immune receptors. Cblb is involved in regulating both innate and adaptive immune cells, and is part of pathways such as CD28 and CTLA-4 signaling [3,6]. Genetic models, like gene knockout mouse models, have been crucial in understanding its functions.
In gene knockout models, Cblb-deficient CD8+ T cells show resistance to regulatory T cells, accompanied by IFNγ induction and down-modulation of TGFβ/SMAD signaling, making them more effective against cancers [2]. In CD4+ T cells, Cblb deficiency leads to hyperproliferation, excessive IL-2 production, and resistance to Treg suppression, overcoming Treg-mediated inhibition at transcriptional and translational levels [4]. Cblb-deficient mice also develop hyper T follicular helper (Tfh) cell responses and lupus due to reduced BCL6 degradation [1]. In addition, homozygous mutations in Cblb in mice cause T and B cell hyperproliferation, and T effector cells are resistant to WT Treg suppression [6]. Ablation of Cblb in human placental stem cell-derived NK cells enhances their cytotoxicity against tumors [5].
In conclusion, Cblb is a significant regulator in the immune system. Through gene knockout mouse models, its role in immune-related diseases such as autoimmunity, lupus, and cancer has been revealed. These findings suggest that targeting Cblb could be a potential therapeutic strategy for these diseases.
References:
1. Li, Xin, Sun, Weili, Huang, Mengxing, Lian, Zhe-Xiong, Gu, Hua. 2024. Deficiency of CBL and CBLB ubiquitin ligases leads to hyper T follicular helper cell responses and lupus by reducing BCL6 degradation. In Immunity, 57, 1603-1617.e7. doi:10.1016/j.immuni.2024.04.023. https://pubmed.ncbi.nlm.nih.gov/38761804/
2. Wolf, Dominik, Baier, Gottfried. . IFNγ Helps CBLB-Deficient CD8+ T Cells to Put Up Resistance to Tregs. In Cancer immunology research, 10, 370. doi:10.1158/2326-6066.CIR-22-0080. https://pubmed.ncbi.nlm.nih.gov/35362047/
3. Augustin, Ryan C, Bao, Riyue, Luke, Jason J. . Targeting Cbl-b in cancer immunotherapy. In Journal for immunotherapy of cancer, 11, . doi:10.1136/jitc-2022-006007. https://pubmed.ncbi.nlm.nih.gov/36750253/
4. Song, Jing, Anderson, Warren, Hu, Alex, Rawlings, David J, Buckner, Jane H. 2022. CBLB Deficiency in Human CD4+ T Cells Results in Resistance to T Regulatory Suppression through Multiple Mechanisms. In Journal of immunology (Baltimore, Md. : 1950), 209, 1260-1271. doi:10.4049/jimmunol.2200219. https://pubmed.ncbi.nlm.nih.gov/36165179/
5. Guo, Xuan, Mahlakõiv, Tanel, Ye, Qian, Hariri, Robert, Zhang, Xiaokui. . CBLB ablation with Nuclease technology enhances cytotoxicity of human placental stem cell-derived NK cells for cancer immunotherapy. In Journal for immunotherapy of cancer, 9, . doi:10.1136/jitc-2020-001975. https://pubmed.ncbi.nlm.nih.gov/33741730/
6. Janssen, Erin, Peters, Zachary, Alosaimi, Mohammed F, Bertoli-Avella, Aida M, Geha, Raif S. 2022. Immune dysregulation caused by homozygous mutations in CBLB. In The Journal of clinical investigation, 132, . doi:10.1172/JCI154487. https://pubmed.ncbi.nlm.nih.gov/36006710/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen