C57BL/6JCya-Stim1em1flox/Cya
Common Name:
Stim1-flox
Product ID:
S-CKO-05362
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Stim1-flox
Strain ID
CKOCMP-20866-Stim1-B6J-VA
Gene Name
Product ID
S-CKO-05362
Gene Alias
SIM
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stim1em1flox/Cya mice (Catalog S-CKO-05362) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033289
NCBI RefSeq
NM_009287
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
STIM1, or stromal interaction molecule 1, is a key component of the store-operated Ca2+ entry (SOCE) pathway. It is located on the endoplasmic reticulum membrane and functions as a Ca2+ sensor of the endoplasmic reticulum Ca2+ store [1,3,4,5,6]. In non-excitable cells, STIM1, along with Orai1 in the plasma membrane, forms the highly Ca2+ selective CRAC channel. When intracellular Ca2+ stores in the endoplasmic reticulum are depleted, STIM1 senses this change and triggers the opening of Orai1 channels, allowing Ca2+ entry. This process is crucial for initiating diverse signalling cascades involved in essential cellular processes like gene regulation, cell growth and death, secretion, and gene transcription [1].
In hepatocellular carcinoma (HCC), STIM1 knockout-HCC cells generated by CRISPR-Cas9 were used to study its role. Results showed that STIM1 was down-regulated in metastatic HCC cells. Low STIM1 levels were associated with poor outcomes for HCC patients. During tumor growth, STIM1 stabilized Snail1 protein through the CaMKII/AKT/GSK-3β pathway, while during metastasis, upregulated Snail1 suppressed STIM1/SOCE. Restoration of STIM1 diminished anoikis-resistance and metastasis induced by Snail1. Mechanistically, downregulated STIM1 shifted the metabolic balance from aerobic glycolysis towards AMPK-activated fatty acid oxidation, contributing to Snail1-driven metastasis and anoikis-resistance [2].
In conclusion, STIM1 is essential for maintaining Ca2+ homeostasis and regulating various cellular functions through the SOCE pathway. Its role in diseases such as HCC, as revealed by gene knockout models, shows that it can act as a metabolic checkpoint regulating tumor invasion and metastasis. Understanding STIM1's functions through model-based research provides insights into the underlying mechanisms of diseases and may offer potential therapeutic targets.
References:
1. Lunz, Victoria, Romanin, Christoph, Frischauf, Irene. 2018. STIM1 activation of Orai1. In Cell calcium, 77, 29-38. doi:10.1016/j.ceca.2018.11.009. https://pubmed.ncbi.nlm.nih.gov/30530091/
2. Zhao, Huakan, Yan, Guifang, Zheng, Lu, Li, Dingshan, Li, Yongsheng. 2020. STIM1 is a metabolic checkpoint regulating the invasion and metastasis of hepatocellular carcinoma. In Theranostics, 10, 6483-6499. doi:10.7150/thno.44025. https://pubmed.ncbi.nlm.nih.gov/32483465/
3. Yan, Cheng-Hao, Bai, Wei-Yu, Zhang, Zhi-Meng, Wang, You-Jun, Sun, Jian-Wei. . The roles and mechanism of STIM1 in tumorigenesis and metastasis. In Yi chuan = Hereditas, 45, 395-408. doi:10.16288/j.yczz.23-035. https://pubmed.ncbi.nlm.nih.gov/37194587/
4. Choi, Seok, Maleth, Jozsef, Jha, Archana, Ahuja, Malini, Muallem, Shmuel. . The TRPCs-STIM1-Orai interaction. In Handbook of experimental pharmacology, 223, 1035-54. doi:10.1007/978-3-319-05161-1_13. https://pubmed.ncbi.nlm.nih.gov/24961979/
5. Ke, Yuepeng, Gannaban, Ritchel, Liu, Junchen, Zhou, Yubin. 2024. STIM1 and lipid interactions at ER-PM contact sites. In American journal of physiology. Cell physiology, 328, C107-C114. doi:10.1152/ajpcell.00634.2024. https://pubmed.ncbi.nlm.nih.gov/39620863/
6. Liu, Panpan, Yang, Zhuli, Wang, Youjun, Sun, Aomin. 2023. Role of STIM1 in the Regulation of Cardiac Energy Substrate Preference. In International journal of molecular sciences, 24, . doi:10.3390/ijms241713188. https://pubmed.ncbi.nlm.nih.gov/37685995/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen