C57BL/6JCya-Stk10em1flox/Cya
Common Name:
Stk10-flox
Product ID:
S-CKO-05367
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Stk10-flox
Strain ID
CKOCMP-20868-Stk10-B6J-VA
Gene Name
Product ID
S-CKO-05367
Gene Alias
Gek1; Lok; mKIAA4026
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stk10em1flox/Cya mice (Catalog S-CKO-05367) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102821
NCBI RefSeq
NM_009288
Target Region
Exon 5~7
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
Stk10, serine-threonine kinase 10, is a member of the STE20/p21-activated kinase (PAK) family. It is predominantly expressed in immune organs and is involved in the activation of ERM proteins, playing essential roles in lymphocyte aggregation and adhesion [3,4,5]. It may also be related to the regulation of ribosome biogenesis and is associated with the polo-like kinase kinase family, potentially regulating Plk1 function [2,6].
In gene knockout studies, Stk10 deficiency in mice promotes tumor growth. This is due to decreased activated/effector cytotoxic T lymphocytes (CTLs) and increased vessel density in the tumor microenvironment of prostate cancer, indicating its role in host anti-tumor response [1]. In cervical cancer cells, knockout of Stk10 promotes cell adhesion, migration, and invasion, while in prostate cancer cells, Stk10 knockout promotes cell proliferation and inhibits migration [4,5]. In acquired pure red cell aplasia, STK10 mutations block erythropoiesis by impairing ribosome biogenesis [2].
In conclusion, Stk10 has diverse biological functions. Through gene knockout mouse models, its importance in tumor-related processes such as tumor growth, cell migration, and invasion has been revealed. Additionally, its role in erythropoiesis-related diseases has also been demonstrated, contributing to our understanding of the underlying mechanisms of these diseases.
References:
1. Ma, Jin-Xia, Xu, Dan-Dan, Lu, Shun-Yuan, Wang, Zhu-Gang, Zhang, Hong-Xin. 2022. Stk10 Deficiency in Mice Promotes Tumor Growth by Dysregulating the Tumor Microenvironment. In Biology, 11, . doi:10.3390/biology11111668. https://pubmed.ncbi.nlm.nih.gov/36421382/
2. Yang, Jichun, Shi, Xiaofeng, Liu, Xinyao, Han, Bing, Long, Zhangbiao. 2024. STK10 mutations block erythropoiesis in acquired pure red cell aplasia via impairing ribosome biogenesis. In Annals of hematology, 103, 2711-2720. doi:10.1007/s00277-024-05802-z. https://pubmed.ncbi.nlm.nih.gov/38761185/
3. Serafim, Ricardo A M, Sorrell, Fiona J, Berger, Benedict-Tilman, Zuercher, William J, Elkins, Jonathan M. 2021. Discovery of a Potent Dual SLK/STK10 Inhibitor Based on a Maleimide Scaffold. In Journal of medicinal chemistry, 64, 13259-13278. doi:10.1021/acs.jmedchem.0c01579. https://pubmed.ncbi.nlm.nih.gov/34463505/
4. Zhang, Lu, Lu, Shun-Yuan, Guo, Rui, Liu, Jie, Zhang, Hong-Xin. . Knockout of STK10 promotes the migration and invasion of cervical cancer cells. In Translational cancer research, 9, 7079-7090. doi:10.21037/tcr-20-1601. https://pubmed.ncbi.nlm.nih.gov/35117313/
5. Zhang, Lu, Lu, Shun-Yuan, Guo, Rui, Wang, Zhu-Gang, Zhang, Hong-Xin. 2021. STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. In Experimental and therapeutic medicine, 22, 851. doi:10.3892/etm.2021.10283. https://pubmed.ncbi.nlm.nih.gov/34149897/
6. Walter, Sarah A, Cutler, Richard E, Martinez, Ricardo, Gishizky, Mikhail, Hill, Ronald J. 2003. Stk10, a new member of the polo-like kinase kinase family highly expressed in hematopoietic tissue. In The Journal of biological chemistry, 278, 18221-8. doi:. https://pubmed.ncbi.nlm.nih.gov/12639966/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen