C57BL/6JCya-Supt5em1flox/Cya
Common Name:
Supt5-flox
Product ID:
S-CKO-05435
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Supt5-flox
Strain ID
CKOCMP-20924-Supt5-B6J-VA
Gene Name
Product ID
S-CKO-05435
Gene Alias
Spt5; Supt5h
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Supt5em1flox/Cya mice (Catalog S-CKO-05435) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003527
NCBI RefSeq
NM_013676
Target Region
Exon 14~16
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Supt5, along with Supt4, is the murine ortholog of the yeast transcriptional elongation factor Spt4/Spt5. These factors play a crucial role in transcriptional elongation, which is an essential process in gene expression, thereby influencing various biological processes [1].
In the context of spinocerebellar ataxia type 36 (SCA36), a disease caused by a hexanucleotide GGCCTG repeat expansion, small interfering RNA-mediated silencing of Supt4a/Supt5 was studied. Supt4a knockdown suppressed the formation of (GGCCUG)exp RNA foci and DPR protein formation via regulation of (GGCCUG)exp mRNA, thus ameliorating cytotoxicity in SCA36 cell models [1].
In conclusion, Supt5, as part of the transcriptional elongation machinery, is vital for normal gene expression. Its knockdown in SCA36 cell models demonstrated potential therapeutic effects by reducing RNA foci and DPR protein formation, suggesting its significance in developing treatments for SCA36 and potentially other repeat-expansion disorders.
References：
1. Furuta, Natsumi, Tsukagoshi, Setsuki, Hirayanagi, Kimitoshi, Ikeda, Yoshio. 2019. Suppression of the yeast elongation factor Spt4 ortholog reduces expanded SCA36 GGCCUG repeat aggregation and cytotoxicity. In Brain research, 1711, 29-40. doi:10.1016/j.brainres.2018.12.045. https://pubmed.ncbi.nlm.nih.gov/30610877/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen