C57BL/6JCya-Swap70em1flox/Cya
Common Name:
Swap70-flox
Product ID:
S-CKO-05459
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Swap70-flox
Strain ID
CKOCMP-20947-Swap70-B6J-VA
Gene Name
Product ID
S-CKO-05459
Gene Alias
70kDa
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Swap70em1flox/Cya mice (Catalog S-CKO-05459) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033325
NCBI RefSeq
NM_009302
Target Region
Exon 6
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Swap70, also known as Switch-associated protein 70, is a guanine nucleotide exchange factor involved in various cellular processes. It can interact with Rho-family GTPases, lipid phosphatidylinositol (3,4)-bisphosphate, and filamentous actin, playing roles in immunoregulation, cell maturation, and cell transformation [2,3,4]. It is also associated with pathways like mitogen-activated protein kinases signaling [1].
In cardiac hypertrophy, Swap70 knockout mice and knockdown cardiomyocytes experiments showed that Swap70 deficiency accelerates the progression of pathological cardiac hypertrophy, while overexpression restrains it. Swap70 suppresses cardiac hypertrophy possibly by inhibiting the mitogen-activated protein kinases signaling pathway in a TAK1-dependent manner [1].
In non-alcoholic fatty liver disease (NAFLD), hepatocyte-specific Swap70 knockout and overexpression mice fed a high-fat, high-cholesterol diet demonstrated that SWAP70 suppressed the progression of non-alcoholic steatohepatitis by inhibiting lipid accumulation, inflammatory response, and fibrosis, also through inhibiting the TAK1-related signaling [5].
In conclusion, Swap70 plays essential roles in multiple biological processes and diseases. The use of gene knockout mouse models has revealed its significance in pathological cardiac hypertrophy and NAFLD, mainly by modulating related signaling pathways, providing insights into the underlying mechanisms of these diseases and potential therapeutic targets.
References:
1. Qian, Qiaofeng, Hu, Fengjiao, Yu, Wenjun, Liang, Chuan, Liu, Jinping. 2023. SWAP70 Overexpression Protects Against Pathological Cardiac Hypertrophy in a TAK1-Dependent Manner. In Journal of the American Heart Association, 12, e028628. doi:10.1161/JAHA.122.028628. https://pubmed.ncbi.nlm.nih.gov/36974751/
2. Baranov, Maksim V, Revelo, Natalia H, Verboogen, Daniëlle R J, Ter Beest, Martin, van den Bogaart, Geert. 2018. SWAP70 is a universal GEF-like adaptor for tethering actin to phagosomes. In Small GTPases, 10, 311-323. doi:10.1080/21541248.2017.1328302. https://pubmed.ncbi.nlm.nih.gov/28489960/
3. Kriplani, Nisha, Duncan, Rory R, Leslie, Nicholas R. 2019. SWAP70 undergoes dynamic conformational regulation at the leading edge of migrating cells. In FEBS letters, 593, 395-405. doi:10.1002/1873-3468.13326. https://pubmed.ncbi.nlm.nih.gov/30636036/
4. Baranov, Maksim V, Revelo, Natalia H, Dingjan, Ilse, Honigmann, Alf, van den Bogaart, Geert. . SWAP70 Organizes the Actin Cytoskeleton and Is Essential for Phagocytosis. In Cell reports, 17, 1518-1531. doi:10.1016/j.celrep.2016.10.021. https://pubmed.ncbi.nlm.nih.gov/27806292/
5. Qian, Qiaofeng, Li, Yang, Fu, Jiajun, Liu, Hui, Liu, Jinping. 2022. Switch-associated protein 70 protects against nonalcoholic fatty liver disease through suppression of TAK1. In Hepatology (Baltimore, Md.), 75, 1507-1522. doi:10.1002/hep.32213. https://pubmed.ncbi.nlm.nih.gov/34689362/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen