C57BL/6JCya-Mettl14em1flox/Cya
Common Name:
Mettl14-flox
Product ID:
S-CKO-05533
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mettl14-flox
Strain ID
CKOCMP-210529-Mettl14-B6J-VA
Gene Name
Product ID
S-CKO-05533
Gene Alias
G430022H21Rik; mKIAA1627
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mettl14em1flox/Cya mice (Catalog S-CKO-05533) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029759
NCBI RefSeq
NM_201638
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
METTL14, known as Methyltransferase-like 14, is a major RNA N6-adenosine methyltransferase. It forms part of the RNA m6A methyltransferase complex, regulating RNA function through m6A modification, which impacts numerous biological processes like cell differentiation, metabolism, and disease development [1-5].
In colorectal cancer, METTL14 expression is downregulated and is an independent prognostic factor. Its deletion promotes tumor growth in mouse models. It inhibits cell migration, invasion, and metastasis by mediating m6A modification of SOX4 mRNA [1]. In p53-wild-type CRC cells, METTL14 is transcriptionally activated by wild-type p53 and suppresses tumor growth by restraining aerobic glycolysis [2].
In diabetic cardiomyopathy, METTL14 is downregulated in cardiomyocytes. Its overexpression suppresses pyroptosis and DCM by downregulating lncRNA TINCR [3].
In endothelial cells, METTL14 promotes FOXO1 expression through m6A modification, aggravating endothelial inflammation and atherosclerosis. METTL14 knockout mice show reduced atherosclerotic plaque development [4].
In hematopoiesis, silencing METTL14 in normal hematopoietic stem/progenitor cells and AML cells promotes terminal myeloid differentiation and inhibits AML cell survival/proliferation [5].
In summary, METTL14 is crucial in multiple disease-related biological processes. Gene knockout mouse models have been instrumental in revealing its roles in diseases such as colorectal cancer, diabetic cardiomyopathy, atherosclerosis, and in hematopoiesis. These findings enhance our understanding of the underlying disease mechanisms and suggest METTL14 as a potential therapeutic target.
References:
1. Chen, Xiaoxiang, Xu, Mu, Xu, Xueni, Sun, Huilin, Wang, Shukui. 2020. METTL14-mediated N6-methyladenosine modification of SOX4 mRNA inhibits tumor metastasis in colorectal cancer. In Molecular cancer, 19, 106. doi:10.1186/s12943-020-01220-7. https://pubmed.ncbi.nlm.nih.gov/32552762/
2. Hou, Yichao, Zhang, Xintian, Yao, Han, Fang, Jing-Yuan, Meng, Xiangjun. 2023. METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cells. In EMBO reports, 24, e56325. doi:10.15252/embr.202256325. https://pubmed.ncbi.nlm.nih.gov/36794620/
3. Meng, Liping, Lin, Hui, Huang, Xingxiao, Peng, Fang, Wu, Shengjie. 2022. METTL14 suppresses pyroptosis and diabetic cardiomyopathy by downregulating TINCR lncRNA. In Cell death & disease, 13, 38. doi:10.1038/s41419-021-04484-z. https://pubmed.ncbi.nlm.nih.gov/35013106/
4. Jian, Dongdong, Wang, Ying, Jian, Liguo, Wang, Shuai, Li, Muwei. 2020. METTL14 aggravates endothelial inflammation and atherosclerosis by increasing FOXO1 N6-methyladeosine modifications. In Theranostics, 10, 8939-8956. doi:10.7150/thno.45178. https://pubmed.ncbi.nlm.nih.gov/32802173/
5. Weng, Hengyou, Huang, Huilin, Wu, Huizhe, He, Chuan, Chen, Jianjun. 2017. METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m6A Modification. In Cell stem cell, 22, 191-205.e9. doi:10.1016/j.stem.2017.11.016. https://pubmed.ncbi.nlm.nih.gov/29290617/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen