C57BL/6JCya-Tsga10em1flox/Cya
Common Name:
Tsga10-flox
Product ID:
S-CKO-05582
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tsga10-flox
Strain ID
CKOCMP-211484-Tsga10-B6J-VA
Gene Name
Product ID
S-CKO-05582
Gene Alias
4933432N21Rik; Gm217; Mtsga10
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tsga10em1flox/Cya mice (Catalog S-CKO-05582) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041815
NCBI RefSeq
NM_207228
Target Region
Exon 7~9
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Tsga10, or testis-specific gene antigen 10, is a protein-coding gene. It is highly conserved among different species and is testis-specific. It has crucial impacts on cancer progression and spermatogenesis [1,2]. In cancer, it may be involved in signaling pathways related to carcinogenesis, and in spermatogenesis, it is associated with the arrangement of mitochondrial sheath in spermatozoa [1,2]. Genetic models like mouse models are valuable for studying its functions.
Tsga10 knockout (KO) mouse models generated by Nuclease technology technology showed that heterozygous Tsga10 male mice were infertile with significantly reduced sperm motility due to disordered mitochondrial sheath formation. This indicates that Tsga10 is essential for the correct arrangement of mitochondrial sheath in spermatozoa, thus being crucial for male fertility [2]. In addition, in cancer-related studies, overexpression of Tsga10 in HeLa cells disrupted the hypoxia-inducible factor-1α (HIF-1α) axis, and in human umbilical vein endothelial cells (HUVECs), it decreased cell proliferation, migration, and in vitro angiogenesis, acting as a tumor suppressor gene. In breast cancer cells, Tsga10 overexpression decreased metastatic and metabolic activity by inhibiting HIF-1 [3,4].
In conclusion, Tsga10 plays essential roles in spermatogenesis, being vital for male fertility through its role in mitochondrial sheath arrangement in spermatozoa. In the context of cancer, it shows potential as a tumor suppressor by affecting cancer cell metabolism, angiogenesis, and metastasis. The use of KO mouse models has been instrumental in revealing these functions, providing insights into male infertility and cancer-related disease areas [1,2,3,4].
References:
1. Taghizadeh-Hesary, Farzad, Ghadyani, Mobina, Kashanchi, Fatah, Behnam, Babak. 2024. Exploring TSGA10 Function: A Crosstalk or Controlling Mechanism in the Signaling Pathway of Carcinogenesis? In Cancers, 16, . doi:10.3390/cancers16173044. https://pubmed.ncbi.nlm.nih.gov/39272902/
2. Luo, Geng, Hou, Meiqi, Wang, Bo, Wang, Jiarui, Zhang, Xianqin. 2020. Tsga10 is essential for arrangement of mitochondrial sheath and male fertility in mice. In Andrology, 9, 368-375. doi:10.1111/andr.12889. https://pubmed.ncbi.nlm.nih.gov/32790169/
3. Amoorahim, Mahtab, Valipour, Elahe, Hoseinkhani, Zohreh, Moradi, Sajad, Mansouri, Kamran. 2019. TSGA10 overexpression inhibits angiogenesis of HUVECs: A HIF-2α biased perspective. In Microvascular research, 128, 103952. doi:10.1016/j.mvr.2019.103952. https://pubmed.ncbi.nlm.nih.gov/31704243/
4. Jahani, Mozhgan, Shahlaei, Mohsen, Norooznezhad, Fatemeh, Mahnam, Azadeh, Mansouri, Kamran. 2020. TSGA10 Over Expression Decreases Metastasic and Metabolic Activity by Inhibiting HIF-1 in Breast Cancer Cells. In Archives of medical research, 51, 41-53. doi:10.1016/j.arcmed.2019.12.002. https://pubmed.ncbi.nlm.nih.gov/32086108/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen