C57BL/6JCya-Tacc3em1flox/Cya
Common Name:
Tacc3-flox
Product ID:
S-CKO-05702
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tacc3-flox
Strain ID
CKOCMP-21335-Tacc3-B6J-VA
Gene Name
Product ID
S-CKO-05702
Gene Alias
Aint; Eric1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tacc3em1flox/Cya mice (Catalog S-CKO-05702) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114426
NCBI RefSeq
NM_001040435
Target Region
Exon 4~5
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Tacc3, a member of the transforming acidic coiled-coil domain-containing protein (TACC) family, is one of the major recruitment factors of distinct multi-protein complexes. It localizes to spindles, centrosomes, and the nucleus, and is crucial for regulating key oncogenic processes like cell proliferation, migration, invasion, and stemness. It also has spatiotemporal functions throughout the cell cycle, and its study in genetic models can help understand its complex roles [1].
In cancer, Tacc3 has been widely studied. High Tacc3 expression is associated with poor prognosis in multiple cancers such as pancreatic cancer [3], lung carcinoma [5]. In breast cancer with centrosome amplification, Tacc3 forms distinct functional interactomes in mitosis and interphase to ensure cancer cell proliferation and survival. In mitosis, it interacts with KIFC1 to cluster extra centrosomes, and in interphase, it interacts with the NuRD complex in the nucleus to inhibit key tumor suppressors. Targeting Tacc3 can lead to mitotic cell death via multipolar spindle formation and G1 arrest and apoptosis [4]. Additionally, in HER2-positive breast cancer, Tacc3 is overexpressed in T-DM1-resistant cells, and its inhibition can restore T-DM1-induced immunogenic cell death and enhance the drug's efficacy [2].
In conclusion, Tacc3 is a multifunctional protein with a significant role in cancer development and progression. Studies, especially those using models to target Tacc3, have revealed its importance in various oncogenic processes. Understanding Tacc3's functions can potentially lead to new therapeutic strategies for treating aggressive cancers.
References:
1. Saatci, Ozge, Sahin, Ozgur. 2024. TACC3: a multi-functional protein promoting cancer cell survival and aggressiveness. In Cell cycle (Georgetown, Tex.), 22, 2637-2655. doi:10.1080/15384101.2024.2302243. https://pubmed.ncbi.nlm.nih.gov/38197196/
2. Gedik, Mustafa Emre, Saatci, Ozge, Oberholtzer, Nathaniel, Mehrotra, Shikhar, Sahin, Ozgur. . Targeting TACC3 Induces Immunogenic Cell Death and Enhances T-DM1 Response in HER2-Positive Breast Cancer. In Cancer research, 84, 1475-1490. doi:10.1158/0008-5472.CAN-23-2812. https://pubmed.ncbi.nlm.nih.gov/38319231/
3. Shi, Saimeng, Guo, Duancheng, Ye, Longyun, Jin, Kaizhou, Wu, Weiding. 2023. Knockdown of TACC3 inhibits tumor cell proliferation and increases chemosensitivity in pancreatic cancer. In Cell death & disease, 14, 778. doi:10.1038/s41419-023-06313-x. https://pubmed.ncbi.nlm.nih.gov/38012214/
4. Saatci, Ozge, Akbulut, Ozge, Cetin, Metin, Uner, Aysegul, Sahin, Ozgur. 2023. Targeting TACC3 represents a novel vulnerability in highly aggressive breast cancers with centrosome amplification. In Cell death and differentiation, 30, 1305-1319. doi:10.1038/s41418-023-01140-1. https://pubmed.ncbi.nlm.nih.gov/36864125/
5. Zhang, Xiangmei, Fan, Xiaoyan, Li, Xin, Zhao, Jidong, He, Dongwei. . Abnormal TACC3 Expression is an Independent Prognostic Biomarker in Lung Carcinoma. In Frontiers in bioscience (Landmark edition), 27, 252. doi:10.31083/j.fbl2708252. https://pubmed.ncbi.nlm.nih.gov/36042187/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen