C57BL/6JCya-Arhgef11em1flox/Cya
Common Name
Arhgef11-flox
Product ID
S-CKO-05728
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-213498-Arhgef11-B6J-VA
When using this mouse strain in a publication, please cite “Arhgef11-flox Mouse (Catalog S-CKO-05728) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Arhgef11-flox
Strain ID
CKOCMP-213498-Arhgef11-B6J-VA
Gene Name
Product ID
S-CKO-05728
Gene Alias
Prg, RhoGEF, mKIAA0380, PDZ-RhoGEF
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000039476
NCBI RefSeq
NM_001003912
Target Region
Exon 2~3
Size of Effective Region
~2.2 kb
Overview of Gene Research
Arhgef11, also known as PDZ-RhoGEF, is a Rho-guanine nucleotide exchange factor. It is a specific guanine nucleotide exchange factor (GEF) for RhoA, playing a crucial role in regulating actin cytoskeleton dynamics. It is involved in multiple pathways such as the β-catenin pathway, Rho-ROCK pathway, and is associated with various biological processes like cell migration, epithelial-mesenchymal transition (EMT), and the maintenance of epithelial tight junctions [1,3,4,5].
In HCC, knockdown of ARHGEF11 inhibited cell proliferation and invasion, and attenuated β-catenin nuclear translocation, repressing EMT and causing cell cycle arrest [1]. In the Dahl salt-sensitive rat, loss of Arhgef11 protected against hypertension-induced renal injury, with early transcriptome/protein changes in the cytoskeleton [2]. Depletion of Arhgef11 in mammalian cells reduced Rho GTPase activity crosstalk, cell protrusion-retraction dynamics, and migration distance [3]. Ablation of Esrp1/2 led to loss of the epithelial isoform of Arhgef11, impairing epithelial tight junction integrity [4].
In conclusion, Arhgef11 is essential for various cellular functions including cell migration, EMT, and maintaining epithelial tight junctions. Research using gene-knockout models, such as in rats, has revealed its significant role in diseases like hepatocellular carcinoma and hypertension-induced renal injury, providing valuable insights into the underlying molecular mechanisms and potential therapeutic targets.
References：
1. Du, Jinpeng, Zhu, Zexin, Xu, Lin, Yuan, Kefei, Zeng, Yong. 2020. ARHGEF11 promotes proliferation and epithelial-mesenchymal transition of hepatocellular carcinoma through activation of β-catenin pathway. In Aging, 12, 20235-20253. doi:10.18632/aging.103772. https://pubmed.ncbi.nlm.nih.gov/33122451/
2. Johnson, Ashley C, Wu, Wenjie, Attipoe, Esinam M, Lindsey, Merry L, Garrett, Michael R. 2020. Loss of Arhgef11 in the Dahl Salt-Sensitive Rat Protects Against Hypertension-Induced Renal Injury. In Hypertension (Dallas, Tex. : 1979), 75, 1012-1024. doi:10.1161/HYPERTENSIONAHA.119.14338. https://pubmed.ncbi.nlm.nih.gov/32148127/
3. Nanda, Suchet, Calderon, Abram, Sachan, Arya, Nalbant, Perihan, Dehmelt, Leif. 2023. Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles. In Nature communications, 14, 8356. doi:10.1038/s41467-023-43875-y. https://pubmed.ncbi.nlm.nih.gov/38102112/
4. Lee, SungKyoung, Cieply, Benjamin, Yang, Yueqin, Chan, Patricia, Carstens, Russ P. . Esrp1-Regulated Splicing of Arhgef11 Isoforms Is Required for Epithelial Tight Junction Integrity. In Cell reports, 25, 2417-2430.e5. doi:10.1016/j.celrep.2018.10.097. https://pubmed.ncbi.nlm.nih.gov/30485810/
5. Jia, Zhen, Johnson, Ashley C, Wang, Xuexiang, Kyle, Patrick B, Garrett, Michael R. 2015. Allelic Variants in Arhgef11 via the Rho-Rock Pathway Are Linked to Epithelial-Mesenchymal Transition and Contributes to Kidney Injury in the Dahl Salt-Sensitive Rat. In PloS one, 10, e0132553. doi:10.1371/journal.pone.0132553. https://pubmed.ncbi.nlm.nih.gov/26172442/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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