C57BL/6JCya-Cntn2em1flox/Cya
Common Name:
Cntn2-flox
Product ID:
S-CKO-05749
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cntn2-flox
Strain ID
CKOCMP-21367-Cntn2-B6J-VA
Gene Name
Product ID
S-CKO-05749
Gene Alias
D130012K04Rik; TAG-1; TAG1; Tax
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cntn2em1flox/Cya mice (Catalog S-CKO-05749) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086521
NCBI RefSeq
NM_177129
Target Region
Exon 3~7
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Cntn2, also known as Contactin-2 or TAG-1, is a neuronal recognition molecule of the immunoglobulin superfamily. It is involved in crucial biological processes such as axon guidance, neuronal migration, and fasciculation [1,2]. It forms transient homophilic interactions, and its structure reveals a T-shaped homodimer [1]. It may be part of pathways related to neural circuit development, and genetic models can be used to study its functions in depth.
In a mouse model where EGFP was expressed under the Tag-1 promoter and Diptheria Toxin subunit A was used to ablate CNTN2+ cortical neurons, significant defects were observed. These included a smaller cortex, reduction of corticothalamic axons, and callosal and commissural defects, suggesting its importance in neocortical size and axonal tract formation during development [2]. In Drosophila, loss of CNTN2, a predicted protective modifier, rescued loss of PIGA in a PIGA-CDG model, as well as patient-relevant phenotypes like seizures and climbing defects, indicating its role as a genetic modifier in this rare congenital disorder [3].
In conclusion, Cntn2 is vital for processes like axon guidance, neuronal migration, and fasciculation. The use of gene-knockout models in mice and Drosophila has revealed its role in neocortical development and as a genetic modifier in PIGA-CDG. These findings contribute to our understanding of normal neural development and the pathogenesis of related disorders.
References:
1. Fan, Shanghua, Liu, Jianfang, Chofflet, Nicolas, Ren, Gang, Rudenko, Gabby. 2024. Molecular mechanism of contactin 2 homophilic interaction. In Structure (London, England : 1993), 32, 1652-1666.e8. doi:10.1016/j.str.2024.06.004. https://pubmed.ncbi.nlm.nih.gov/38968938/
2. Kastriti, Maria Eleni, Stratigi, Aikaterini, Mariatos, Dimitris, Adameyko, Igor, Karagogeos, Domna. 2019. Ablation of CNTN2+ Pyramidal Neurons During Development Results in Defects in Neocortical Size and Axonal Tract Formation. In Frontiers in cellular neuroscience, 13, 454. doi:10.3389/fncel.2019.00454. https://pubmed.ncbi.nlm.nih.gov/31749685/
3. Thorpe, Holly J, Pedersen, Brent S, Dietze, Miranda, Thomas, Ashley, Chow, Clement Y. 2024. Identification of CNTN2 as a genetic modifier of PIGA-CDG through pedigree analysis of a family with incomplete penetrance and functional testing in Drosophila. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.08.12.607501. https://pubmed.ncbi.nlm.nih.gov/39211166/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen