C57BL/6JCya-Zeb1em1flox/Cya
Common Name:
Zeb1-flox
Product ID:
S-CKO-05810
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zeb1-flox
Strain ID
CKOCMP-21417-Zeb1-B6J-VA
Gene Name
Product ID
S-CKO-05810
Gene Alias
3110032K11Rik; AREB6; BZP; MEB1; Nil2; TCF-8; Tcf18; Tcf8; Tw; ZEB; Zfhep; Zfhx1a; Zfx1a; Zfx1ha; [delta]EF1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zeb1em1flox/Cya mice (Catalog S-CKO-05810) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025081
NCBI RefSeq
NM_011546
Target Region
Exon 6
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Zeb1, also known as zinc finger E-box binding homeobox 1, is a key transcription factor in epithelial-mesenchymal transition (EMT). EMT is a process where epithelial cells acquire mesenchymal characteristics, which is involved in numerous biological processes like embryonic development, fibrosis, and tumor progression [4,5]. Zeb1 is associated with multiple signaling pathways, such as those related to tumor metabolism and immune escape. Genetic models, especially gene knockout (KO) or conditional knockout (CKO) mouse models, are valuable for studying Zeb1's functions.
In cancer research, multiple studies have revealed Zeb1's significance. In osteosarcoma, miR-144-3p can promote ferroptosis and inhibit tumor cell proliferation, migration, and invasion by negatively regulating Zeb1 expression [1]. In hepatocellular carcinoma (HCC), Zeb1 transcriptionally activates PFKM, enhancing the Warburg effect and facilitating tumorigenesis and metastasis. Knockdown of Zeb1 in HCC cell lines leads to impaired glycolysis, proliferation, and invasion, which can be rescued by exogenous PFKM expression [2]. In breast cancer, Zeb1 promotes the Warburg effect by increasing the transcriptional expression of glycolytic rate-determining enzymes, and also induces M2 macrophage polarization, contributing to an immunosuppressive tumor microenvironment [3].
In conclusion, Zeb1 plays essential roles in EMT-related biological processes and is closely associated with cancer development. The use of KO/CKO mouse models in these studies has provided insights into how Zeb1 influences tumor-related metabolic reprogramming, immune escape, and cell-cell interactions in the tumor microenvironment. Understanding Zeb1's functions can potentially offer new therapeutic strategies for cancer treatment.
References:
1. Jiang, Mingyang, Jike, Yiji, Liu, Kaicheng, Qiu, Yue, Bo, Zhandong. 2023. Exosome-mediated miR-144-3p promotes ferroptosis to inhibit osteosarcoma proliferation, migration, and invasion through regulating ZEB1. In Molecular cancer, 22, 113. doi:10.1186/s12943-023-01804-z. https://pubmed.ncbi.nlm.nih.gov/37461104/
2. Zhou, Yanming, Lin, Furong, Wan, Tao, Lin, Donghai, Li, Qinxi. 2021. ZEB1 enhances Warburg effect to facilitate tumorigenesis and metastasis of HCC by transcriptionally activating PFKM. In Theranostics, 11, 5926-5938. doi:10.7150/thno.56490. https://pubmed.ncbi.nlm.nih.gov/33897890/
3. Jiang, Huimin, Wei, Huimin, Wang, Hang, Zhao, Li, Yang, Shuang. 2022. Zeb1-induced metabolic reprogramming of glycolysis is essential for macrophage polarization in breast cancer. In Cell death & disease, 13, 206. doi:10.1038/s41419-022-04632-z. https://pubmed.ncbi.nlm.nih.gov/35246504/
4. Lu, Jiahui, Fei, Fei, Wu, Chenxi, Xu, Junying, Lu, Peihua. 2022. ZEB1: Catalyst of immune escape during tumor metastasis. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 153, 113490. doi:10.1016/j.biopha.2022.113490. https://pubmed.ncbi.nlm.nih.gov/36076506/
5. Cheng, Lin, Zhou, Ming-Yuan, Gu, Ying-Jian, Chen, Lei, Wang, Yun. 2021. ZEB1: New advances in fibrosis and cancer. In Molecular and cellular biochemistry, 476, 1643-1650. doi:10.1007/s11010-020-04036-7. https://pubmed.ncbi.nlm.nih.gov/33417164/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen