C57BL/6JCya-Tfebem1flox/Cya
Common Name:
Tfeb-flox
Product ID:
S-CKO-05821
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tfeb-flox
Strain ID
CKOCMP-21425-Tfeb-B6J-VA
Gene Name
Product ID
S-CKO-05821
Gene Alias
Tcfeb; bHLHe35
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tfebem1flox/Cya mice (Catalog S-CKO-05821) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086932
NCBI RefSeq
NM_001161722
Target Region
Exon 2~6
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
TFEB, the transcription factor EB, is a master regulator in the autophagy/lysosomal-to-nucleus signaling pathway, belonging to the MiT/TFE family of basic helix-loop-helix leucine zipper transcription factors [4]. It plays a pivotal role in regulating basic cellular processes such as lysosomal biogenesis and autophagy. TFEB's activity is closely associated with multiple signaling pathways, including mTOR, Wnt, calcium, and AKT, and is crucial for energy homeostasis, stress response, and metabolism [4].
In vivo studies, potentially including KO/CKO mouse models though not explicitly detailed in the provided abstracts, have shown that TFEB is involved in lipid catabolism [1]. Also, TFEB has been found to be capable of inducing the intracellular clearance of pathogenic factors in murine models of diseases like Parkinson's and Alzheimer's, suggesting its potential in developing novel therapeutic strategies [1]. In autophagy, TFEB coordinates a transcriptional program controlling autophagosome formation, autophagosome-lysosome fusion, and substrate degradation during starvation, by driving the expression of autophagy and lysosomal genes [2]. The ubiquitin-proteasome pathway, through the E3 ubiquitin ligase STUB1, modulates TFEB activity, affecting autophagy and mitochondrial biogenesis [3].
In conclusion, TFEB is a central regulator of lysosomal biogenesis, autophagy, and other cellular processes. Model-based research, potentially including KO/CKO mouse models, has revealed its significance in neurodegenerative diseases, lipid catabolism, and autophagy-related functions. Understanding TFEB's role provides insights into disease mechanisms and potential therapeutic targets for neurodegenerative and other related diseases.
References:
1. Napolitano, Gennaro, Ballabio, Andrea. 2016. TFEB at a glance. In Journal of cell science, 129, 2475-81. doi:10.1242/jcs.146365. https://pubmed.ncbi.nlm.nih.gov/27252382/
2. Settembre, Carmine, Di Malta, Chiara, Polito, Vinicia Assunta, Rubinsztein, David C, Ballabio, Andrea. 2011. TFEB links autophagy to lysosomal biogenesis. In Science (New York, N.Y.), 332, 1429-33. doi:10.1126/science.1204592. https://pubmed.ncbi.nlm.nih.gov/21617040/
3. Sha, Youbao, Rao, Lang, Settembre, Carmine, Ballabio, Andrea, Eissa, N Tony. 2017. STUB1 regulates TFEB-induced autophagy-lysosome pathway. In The EMBO journal, 36, 2544-2552. doi:10.15252/embj.201796699. https://pubmed.ncbi.nlm.nih.gov/28754656/
4. Tan, Anderson, Prasad, Renuka, Lee, Chaerin, Jho, Eek-Hoon. 2022. Past, present, and future perspectives of transcription factor EB (TFEB): mechanisms of regulation and association with disease. In Cell death and differentiation, 29, 1433-1449. doi:10.1038/s41418-022-01028-6. https://pubmed.ncbi.nlm.nih.gov/35739255/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen